中文摘要：

建立一种基于纳米金生物条形码技术的二噁英快速筛检方法．利用二噁英诱导激活的芳香烃受体复合物，特异识别以纳米金为报告基团的二噁英反应探针，该探针被释放后进行务形码放大，通过纳米金银染技术增强识别信号，并记录其吸光度值，从而可以简单灵敏地快速筛检二噁英类化合物．在一定的反应时间和浓度范围内（10^-14～10^-10 mol／L），溶液的吸光度值与2，3，7，8-四氯二苯并二噁英（2，3，7，8-tetrachlorodibenzo-p-dioxin，TCDD）浓度之间呈正相关，方法检测限为0．01pmol／L，变异系数为5％～8％．用纳米金生物条形码（Nano-Barcode，nanopartiele-based bio-barcode）方法和现有的生物分析方法（CALUX，chemical-activated lueiferase gene expression）分别测定TCDD标准品，并绘制剂量效应曲线．结果表明，本方法灵敏度高，线性范围宽，重复性好．本研究纳米金生物条形码方法的检测灵敏度高于CALUX将近5倍（EC50分别为4×10^-12 mol／L和2×10^-11 mol／L），检测限较CALUX降低了10倍（检测限分别为1×10^-14 mol／L和1×10^-13mol／L），变异系数分别为5％～8％和15％～30％．

英文摘要：

We have established a nannparticle-based bio-barcode method for the prescreening of dioxins. The formation of AhR-Amt complex triggered by dioxin resulted in a high-affinity to DRE. The DRE probes were completely released as bio-barcodes and amplified by nanoparticle based silver enhancement, then the absorbance of TCDD（2,3,7,8- tetrachlorodibenzo-p-dioxin） were recorded as quantitative signals. The dose- dependency of the assay range between 10^-14mol/L to 10^-10mol/L for soluable products. The TCDD detection limit was 0.01 pmol/L with a coefficient of variation as 5 %-8 % . Compared with CALUX（ chemical-activated luciferase gene expression） bioassay, the sensitivity of our Nano-Barcode method was approximately 5 times higher with ECso of 4×10^-12 mol/L, and the detection limit was 10 times lower as 1×10^-14 mol/L. In addition, our method exhibited a better reproducibility of CV as 5 %-8 % compared to 15 %-30 % of CALUX.