中文摘要：

编码 polygalacturonase-inhibitor-like 蛋白质(PGIP ) 的 cDNA 从棉花花 cDNA 图书馆被孤立。cDNA，指明的 GhPS1 (GenBank 同意没有。ABO47744 ) ，与与已知的植物 PGIP 分享高类似的 370 氨基酸编码蛋白质。荧光灯显微镜学显示那 GhPS1 蛋白质在房间膜上本地化象一样在里面细胞质。实时量的 RTPCR 和北污点分析证明 GhPS1 明确地在棉花花瓣被表示。而且， GhPS1 表示在花瓣开发是逐渐地起来调整的，并且它的抄本在开花期在花瓣被积累到高水平。然而，它的表示水平在 flowering 以后在开始衰老的花瓣很快被拒绝。在低温度， GhPS1 基因表示逐渐地被减少到在花瓣很低级。一起，我们的结果建议那 GhPS1 基因可能涉及棉花花瓣开发和老朽，并且响应寒冷强调。

英文摘要：

A cDNA encoding a polygalacturonase-inhibitor-like protein （PGIP） was isolated from cotton flower cDNA library. The cDNA, designated GhPS1 （GenBank accession No. ABO47744）, encodes a protein with 370 amino acids that shares high similarity with the known plant PGIPs. Fluorescent microscopy indicated that GhPS1 protein localizes on the cell membranes as well as in cytoplasm. Real-time quantitative RT-PCR and northern blot analyses showed that GhPS1 was specifically expressed in cotton petals. Furthermore, the GhPS1 expression was gradually up-regulated in petal development, and its transcripts were accumulated to the highest level in the petals at anthesis. However, its expression level was declined rapidly in senesced petals after flowering. At low temperature, the GhPS1 gene expression was gradually decreased to very low level in petals. Collectively, our results suggest that GhPSI gene might be involved in cotton petal development and senescence, and in response to cold stress.