中文摘要：

以负载1,4,7,10-四氮杂环十二烷（cyclen）的氯甲基壳聚糖树脂（merrifield chitosan resin-supported cyclen,MCRC）为载体,配合Ce（Ⅲ）,得到以Ce（Ⅲ）为催化中心的改性氯甲基壳聚糖Ce（Ⅲ）树脂（MCRC-Ce（Ⅲ））。通过茚三酮比色法、SDS-PAGE电泳法及氨基酸自动分析仪等技术,检测MCRC-Ce（Ⅲ）对牛血清蛋白（BSA）的水解能力。结果表明,MCRCCe（Ⅲ）在生理pH值,60℃条件下,可将BSA水解为短肽和氨基酸,24 h后水解率可达88%,水解速率表观常数Kobs为7.05×10-2mol·L-1·h-1,重复使用6次后,仍能保持80%以上的水解能力,具有广阔的应用前景。

英文摘要：

Merrifield chitosan resin-supported cyclen （MCRC）was prepared by attaching 1,4,7,10-tetraazacy-clododecane （cyclen）to Merrifield chitosan resin （MCR）.Then coordination with Ce（Ⅲ）as catalytic center to be MCRC-Ce（Ⅲ）.Ninhydrin reaction,SDS-PAGE,as well as automatic amino-acid analyzor were drawn to in-vestigate the hydrolyzing ability of MCRC-Ce（Ⅲ）toward BSA.The results indicated that after 24 hours,BSA can be hydrolyzed up to 88% at physiological pH,60℃,where protein was hydrolyzed into smaller peptides and amino acids,and the pseudo-first-order kinetic constant （K obs ）was 7.05×10-2mol·L-1·h-1 .MCRC-Ce（Ⅲ） has a strong binding affinity with metal ions,which could be reused for 6 times while the catalytic ability main-tains 80% of the original,indicating that MCRC-Ce（Ⅲ）has a broad application prospect.