中文摘要：

目的探讨安徽省既往献血的HIV慢性感染人群血浆中和能力及其与CD4、病毒载量的关系。方法从安徽省既往献血的294位HIV慢性感染人群静脉抽血，EDTA抗凝后进行血浆和外周血单个核细胞（PBMC）的分离、CD4和病毒载量的检测，采用拥有共享序列的临床分离病毒1597株和实验室株SF33病毒两种病毒分别做血浆的中和试验。用200TCID50病毒感染TZM-bl细胞，通过加入不同稀释度血浆，用Luciferase Assay System检测血浆的中和能力并计算中和50％的病毒进入细胞的血浆浓度（EC50）。结果294位患者血浆针对HIV-1实验株SF33和临床分离株1597的中和能力间差异有统计学意义（P〈0．05），针对实验株的中和能力明显高于临床分离株，91％患者血浆对HIV-1实验株SF33具有一定的中和抗体，其中EC50〉500的抗体水平较高的血浆占33％，对临床分离株1597的中和抗体阳性率为79％，其中EC50〉500的占7％。方差分析显示，血浆中和能力在CD4不同水平之间没有显著差异。294位HIV-1B′亚型感染的血浆病毒载量（lg）平均为4．40（2．60～6．46）。方差分析显示，血浆对SF33的中和能力在不同病毒载量水平之间有显著差异，病毒载量不同的各组血浆针对1597病毒的中和能力之间差异无统计学意义。经Spearman’s相关分析，对SF33株的中和水平与病毒载量呈正相关，对1597株的中和水平与病毒载量的相关性没有统计学意义，在病毒载量lg〈4的患者中，10．53％血浆样品的EC50高于500；在病毒载量lg≥5的患者中，6．46％血浆样品的EC50高于500。结论HIV-1慢性感染人群的血浆对实验株SF33的中和能力随着病毒复制能力的提高而增加，但对临床分离株1597的中和能力未见相关性。

英文摘要：

objective To explore the association among viral load,CD4 count and neutralizing activity of plasma from chronic HIV-1 infected former blood donors in Anhui province, China. Methods 294 chronically HIV-1 B＇ infected individuals from former blood donors in Anhui province were enrolled, whose plasma and peripheral blood mononuclear cells （PBMC） were isolated. The viral load and CD4 were detected, and the neutralization activity of plasma against primary virus（ 1597 ） and lab-adapted strain（ SF33 ） was detected by Luciferase Assay System based on TZM cell line, and 50% neutralizing level was calculated by the Reed-Muench method. Results Neutralizing activity responding against SF33 strain was higher than 1597 （P 〈 0.05 ）. There was a positive correlation between neutralizing concentration of plasma against SF33 and viral load （lg） （r = 0. 191, P = 0. 001 ）, but there was no correlation between neutralizing concentration of plasma against 1597 and viral load（lg）（r =0. 097, P =0. 096 ）. Conclusion In chronic HIV-1 infectious people, neutralizing level against SF33 increases with viral replication, however, there is no association of plasma neutralizing against 1597 replication with viral load.