中文摘要：

使用5＇ LongSAGE标签序列确定了一个新的西方蜜蜂Ypsilon Schachtel（Yps）基因的转录起始位点，并进而预测了该基因的启动子序列．5＇ LongSAGE标签的蜜蜂基因组定位结果表明：在成年雄蜂的头部中，蜜蜂Yps基因存在23个转录起始位点，其中优势转录起始位点有4个，其起始频率分别为27．9％、23．1％、13．5％和11．5％．Yps基因TSS的碱基组成分析发现，此23个TSS第一个碱基为A、G、T、C的概率分别为52％、39％、4%、4％．这些结果暗示RNA聚合酶和调控因子在Yps基因启动子的一个较宽范围内的互作控制着不同转录本的起始效率．

英文摘要：

In this investigation, we determined the transcription initiation start site （TSSs） of a novel honeybee Ypsilon Schachtel（Yps）Yps gene by mapping 5＇ LongSAGE tag sequences to genome sequence of Apis rnellifera. We also analyzed the gene structure as well as promoter sequences of Yps gene. A total of 23 5＇ LongSAGE tags were mapped to upstream of Yps gene start condon, indicating Yps gene can initiate transcription at 23 different sites. Noticeably, there were 75% of bee Yps mRNA were started at 4 dominant TSS （27.9% ,23.1%,13.5% and 11.5%） in adult drone head. The results imply that the interplay between polymerases and regulators over broad promoter region dictates the efficiency of productive transcription for the bee Yps gene. This is the first report about divergent TSSs for bee genes.