中文摘要：

黄瓜花发育的早期均有雌蕊和雄蕊原基的分化，但在发育过程中，由于雌蕊或雄蕊的发育受到阻滞，导致雄花和雌花的形成。近年来在拟南芥和金鱼草等植物中遗传学的研究表明，花器官的特征是由同源异形基因决定的。在拟南芥中，由于ＡＧ在决定雄蕊和雌蕊特征方面起重要作用，本研究利用 ＲＴ－ＰＣＲ技术，从黄瓜的雌、雄蕊中分离出 ＡＧ的同源基因，并对其在花发育过程中的表达和可能作用进行了分析。首先，根据ＡＧ同源基因的保守区域设计５’简并引物５’－ＧＡ（Ａ／Ｇ）ＡＴ（Ｔ／Ｃ／Ａ）ＡＡ（Ｔ／Ｃ／Ａ）ＡＡ（Ｇ／Ａ）（Ａ／Ｃ）Ｇ（Ｇ／Ｔ／Ｃ）ＡＴＣＧＡ（Ｃ／Ａ）ＡＡＣ－３’，然后进行３’，ＲＡ ＣＥ ＰＣＲ，扩增出约１ｋｂ大小的片段，序列分析表明该片段含有非常保守的ＭＡＤＳ ｂｏｘ。进而，利用５’ ＲＡＣＥ ＰＣＲ得到全长度的ｃＤＮＡ。该ｃＤＮＡ的核苷酸序列与ＣＵＭ１（黄瓜 ＭＡＤＳ ｂｏｘ ｇｅｎｅ１）同源性高达９７％。 ＣＵＭ１在接牵牛中过量表达可引起花萼变为心皮状和花瓣变为雄蕊，说明ＣＵＭ１为ＡＧ的同源基因。基于该基因与ＣＵＭ１序列上的高度同源，我们认为其为黄瓜的ＡＧ同源基因。该基因命名为ＣＭＢ１，基因银行登记号为ＡＦ２８６６４９。Ｓｏｕｔｈｅｒ

英文摘要：

As AGAMOUS (AG) homologs are involved in determining the identity of sex organs, we have isolated an AG honolog from sex organs of cucumber by using RT-PCR. This gene was designated as CMBI. Southern analysis indicated that CMBI was present as a single-copy sequence in me genome of cucumber. Northern bybridizatiou demonstrated that CMBI mRNA accumulated in the stamens and pistils at anthesis of both male and female nowers, and mRNA levels in the stamens was much higher than that in the pistils. CMBI mRNA was not detected in the floral organ suck as petals, nor in vegetative organs which include stems and leaves. Furthermore, no signal was detect6d in the young aborted pistiles, suggesting that the activity of CMBI may be required for the normal pistils development in the male flowers.