为了探索呼吸作用在干旱胁迫下如何变化以及外源丙酮酸和琥珀酸对呼吸的调控作用,以欧李Cerasushumili为材料,研究了干旱胁迫条件下外源丙酮酸和琥珀酸对植株叶片含水量、呼吸参数和泛醌氧化还原状态的影响。结果表明:在干旱情况下,抗水杨基羟肟酸(salicyldydraxamic acid,SHAM)呼吸即细胞色素(cytochrome,Cyt)途径呼吸与对照植株相比明显下降,总呼吸和抗氰呼吸即SHAM敏感、交替(alternative,Alt)途径呼吸均有所增加。外源丙酮酸和琥珀酸能够显著刺激呼吸作用并能增加Alt呼吸在总呼吸中的比重,在干旱处理第7天和第14天时,二者对呼吸的刺激作用分别达到最大水平。丙酮酸和琥珀酸能够增加泛醌库还原态水平。推测外源丙酮酸和琥珀酸能降低植物体对外界环境的敏感度,提高植物体应对干旱胁迫的能力。
In order to probe change of respiratory under drought stress and regulation of exogenous pyruvate and succinate on respiration,taking Cerasus humilis as material,effects of exogenous pyruvate and succinate on water content in leaf,respiration parameters,ubiquinone pool status were researched.The results showed that under drought stress,salicyldydraxamic acid(SHAM)-resistant cytochrome(Cyt) pathway reapiration was decreased obviously compared with control plants,both total respiration and the KCN-resistant SHAM-sensitive alternative(Alt) pathway became increasingly engaged.It was found that exogenous pyruvate and succinate drastically enhanced respiration and increased the rate of Alt pathway respiration after drought treatment for 7 and 14 days respectivelly.The reduction level of ubiquinone pool(UQr/UQt) was increased in the plants applied pyruvate or succinate,so exogenous pyruvate and succinate could decrease the sensibility to drought tolerence in C.humilis,and increase the capacity of tolerence to dehydration.