中文摘要：

本研究主要探索枯草芽孢杆菌对仔猪小肠局部天然免疫及TLR表达的影响。选取4窝相同胎次的新生仔猪（15头）为研究对象。分别于仔猪0、7、14、26日龄灌喂枯草芽孢杆菌Bacillus subtilis（活菌数为1×108CFU.mL-1）。试验结果显示：枯草芽孢杆菌能够显著地促进十二指肠中TLR9和细胞因子IL-6的表达（P〈0.05）以及回肠中细胞因子IL-1的表达（P〈0.01）,同时能够显著地提高仔猪小肠IgA分泌细胞的数量（P〈0.01）。结果提示,枯草芽孢杆菌可能通过胞外成分激活小肠TLR9的表达后,诱导细胞因子IL-6的分泌,促进IgA分泌细胞数量增加。饲喂枯草芽孢杆菌具有促进新生仔猪小肠细胞免疫和体液免疫的作用,对预防新生仔猪腹泻提供了应用前景。

英文摘要：

This experiment was conducted to study the effects of Bacillus subtilis on small intestine local mucosal immune responses and the expression of TLRs of pigs.The newborn piglets were orally administrated with Bacillus subtilis at 0,7,14,and 26 d after birth.The results showed that Bacillus subtilis could significantly promote the expression of TLR 9 and cytokine IL-6 in duodenum（P0.05） and increase the quantity of IgA excreting cells（P0.01）,as well as increasing the expression of IL-1 in ileum（P0.01）.The results implied that Bacillus subtilis may increase the expression of IL-6 and the quantity of IgA excreting cells through interact with TLR 9.Orally administrated with Bacillus subtilis to prevent the newborn piglet diarrhea is promising.