中文摘要：

探讨polyI：C是否影响小鼠蜕膜基质细胞存活及其细胞因子的产生，为进一步阐明双链RNA或病毒诱导早孕期母体流产的潜在机制奠定基础。分离培养小鼠蜕膜基质细胞，polyI：C处理细胞24h后，采用噻唑盐比色法（MTT）检测细胞存活率，AnnexinVFITC流式细胞术（FCM）分析polyI：C诱导细胞的死亡方式，以及应用实时定量PCR分析polyI：C处理后IFN-β的表达情况。MTT检测结果表明2mg／LpolyI：C处理细胞后，细胞存活率显著降低（P〈0．01），AnnexinVFITC流式检测说明polyI：C诱导蜕膜基质细胞的细胞死亡方式以坏死和晚期凋亡为主，并且polyI：C可显著促进IFN-β的表达。提示双链RNA能显著促进小鼠蜕膜基质细胞的死亡和细胞因子IFN-β的表达。

英文摘要：

Whether polyI：C affect the cell viability and cytokine production of mouse decidual stromal cells were discussed, which would lay a solid foundation for further understanding the potential mechanism of dsRNA or dsRNA virus-induced early pregnancy miscarriage. The mouse decidual stromal cells were isolat- ed and cultured. Cell survival rate were detected using MTT analysis after the cells were treated by polyI：C for 24 h, the modes of cell death were detected using Annexin V-FITC/PI staining and expression of IFN-β was tested using real-time PCR. MTT assay showed that the survival rate of stromal cells decreased notablely after ceils were treated with 2 mg/L polyI：C（P〈0.01）. Annexin V-FITC/PI testing indicated that the modes of cell death were mainly necrosis and late apoptosis. Also, the expression of IFN-β induced by polyI：C chal- lenge was significantly increased. It suggested that double-strand RNA could promote the cell death of mouse decidual stromal ceils and expression of IFN-β.