中文摘要：

采用高压静电纺丝技术构建了聚氨酯（PU）取向纳米纤维聚合物膜，研究了其引导人脐静脉内皮细胞（HUVEC）生长的作用．通过扫描电子显微镜对PU取向纳米纤维聚合物膜的形貌进行了观察；通过细胞增殖试验，研究了PU取向纳米纤维聚合物膜对HUVEC生长的促进作用；通过激光扫描共聚焦显微镜观察细胞骨架中肌动蛋白、微管蛋白及纽蛋白纤维的形成情况，探讨了取向纳米纤维聚合物膜对细胞迁移、骨架发育的影响．此外，还通过ELESA方法检测了生长在不同聚合物膜上的HUVEC分泌组织因子（TF）的数量，探讨了取向纳米纤维结构对HUVEC抗凝血功能的影响．实验结果表明，PU取向纳米纤维聚合物膜取向良好，直径为300～500nm；该薄膜可明显促进HUVEC增殖；引导HUVEC沿纺丝方向定向排列生长且呈抗凝血表型，组织因子分泌量明显低于对照组PU光滑膜．因此，PU取向纳米纤维聚合物膜可提供适合内皮细胞的良好生存与增殖环境，在血管的修复与再生方面具有潜在的重要应用价值．

英文摘要：

The nanofibrous film of polyurethane （PU） with aligned topography was fabricated by electrospinning for human umbilical vein endothelial cells （HUVEC） growth. The morphology of nanofibrous film was observed and characterized by scanning electron microscopy （SEM）. The cells growth behavior including proliferation, cytoskeleton formation of actin, tublin and vinculin, and tissue factor （TF） release was investigated via cell viability assay, confocal observation and TF assay. The average diameter of the generated fiber was around 300-500 nm. The experimental results indicate that the aligned nanofibrous film of PU exhibited promotional influence on the cell proliferation. It was also observed that the film possessed an advantage of supporting HUVEC migrating and aggregating along the axis of the aligned nanofibers, which is one of the important functions in the process of endothelium regeneration. It was also demonstrated that the endothelial cells growing on the film expressed non-thrombogenic phenotype with low tissue factor released. These results indicate the favorable interactions between ECs and the film, implying that the aligned nanofibrous film of PU has a promising potential for vascular engineering.