中文摘要：

在pH =7.4的Tris-HCl缓冲溶液中,采用荧光光谱法、黏度法等分析方法研究了4-苄氧基苯酚（PBP）与鲱鱼精DNA的作用方式,用摩尔比法确定了PBP与DNA的结合比为2∶1.通过热力学研究得出,在27℃时PBP与鲱鱼精DNA之间相互作用的结合常数为Kθ300.15K=2.16×10^5L/mol,热力学函数ΔrHθm=-6.18×10^4 J/mol,ΔrSθm=-1.05×10^3J/ （mol·K）,ΔrGmθ300.15K=-3.04×10^4 J/mol,结果表明该反应为焓驱动.综合实验结果确定了PBP与DNA的作用方式为部分嵌插以及静电作用.

英文摘要：

The mechanism of interaction between 4-Benzyloxyphenol （PBP） and herring sperm DNA was studied using fluorescence spectroscopy and viscosity analysis in a Tris-HCl solution （ pH = 7.4）. Using mole ration method, the mole ration between PBP and DNA was determined to be 2. Using the thermodynamic analysis, the binding constant of PBP interaction with DNA was determined to be Kθ 300.15k= 2. 16 × 10^5 L/mol, and the corresponding thermodynamic parameters were △rHθm = -6. 18 × 10^4 J/ mol, △rSrm= - 1.05 × 10^3J/ （ mol · K） , △rGmθm300.15K=- 3.04 × 10^4 J/tool. The result suggested that enthalpy was the driven force in the reaction. The interaction of PBP and DNA was derived from partial intercalation and electrostatic interaction.