中文摘要：

目的观察自行研制合成的骨形成蛋白2（BMP2）活性多肽与Ⅰ型胶原复合煅烧骨复合材料修复骨缺损的作用。方法实验分3组。A组：单纯煅烧骨组；B组：含Ⅰ型胶原的煅烧骨组；C组：BMP2活性多肽与Ⅰ型胶原复合煅烧骨组。3组材料植入前分别电镜扫描观察。取大白兔36只，随机分成3组，建立桡骨中段10mm骨缺损模型，分别将材料植入兔桡骨缺损处，术后4、8、12周行X线评分，8、12周行组织形态学观察与评分，比较各组植入材料的成骨作用以及取材后抗折强度。结果36只大白兔均进入结果分析，（1）复合材料电镜扫描结果：孔隙率高，复合结构紧密；（2）术后不同时间X线评分示C组与A组和B组差异有统计学意义（P〈0．05）；（3）组织学观察，8周时评分为：A组4．67±0．52、B组5．33±0．52、C组6．83±0．41。12周评分为：A组评分为6．17±0．41、B组为7．33±0．52、C组为8．67±0．52；（4）各组植人材料抗折强度的测定：术后12周，C组材料抗折强度明显高于A组和B组。结论单纯煅烧骨及复合材料组均有修复骨缺损的能力，但以复合BMP2活性多肽的含Ⅰ型胶原的煅烧骨较为理想。

英文摘要：

Objective To observe the repair of bone defect with sintered bovine cancellous bone complexed with synthetized BMP2-derived peptide and collagen I. Methods Rabbits were divided into 3 groups. Sintered bovine bone was implanted in group A. Sintered bovine bone eomplexed with collagen I was implanted in group B. Sintered bovine bone complexed with BMP2-derived peptide/collagen I was im- planted in group C. Before implantation, the materials were observed under the environmental scanning electron microscopy （ESEM）. Thirty-six rabbits were gained to establish models of 10-mm middle radius bone defects and divided into 3 groups with 12 in each group. Postoperatively, the formed new bone was compared among the three implants at the 4th,8th, 12th week by X ray score, and at the 8th, 12th week by histological score. The bend intensity of implanted materials at the 12th week were tested,respectively. Results Thirty-six rabbits were all involved in the result analysis. （ 1 ） ESEM revealed that the porosity of compound materials was high with close compound. （2） At different postoperative time points, the X ray scores on the group C were significantly greater than in groups A and B （ P 〈 0.05 ）. （3） The histological scores at the 4th and 8th week on the group C were 6.83 ± 0.41 abnd 8.67 ± 0.52 respectively, significantly higher than in both group A （4.67 ± 0.52,6.17±0.41 ） and group B （5.33 ± 0. 52,7.33 ±0.52） respectively （P 〈 0.05 ）. （4） The bend intensity of implanted materials in group C at the 12th week after operation was higher significantly than that in groups A and B. Conclusion Sintered bovine cancellous bone and its compound materials can repair the bone defect, but the sintered bovine bone complexed with BMP2-derived peptide/collagen I is the most ideal.