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慢性乙型肝炎病毒感染者程序性死亡因子1基因拷贝数和mRNA的表达水平

ISSN号：1007-3418
期刊名称：《中华肝脏病杂志》
时间：0
分类：R617[医药卫生—临床医学;医药卫生—外科学]
作者机构：[1]广州市第八人民医院肝病研究所,510060
相关基金：广州市医药卫生科技重点项目（2007-ZDi-04）;广东省产业技术研究与开发资金计划项目（20078060401072）

作者：杨湛[1], 吴令杰[1], 范慧敏[1], 胡凤玉[1], 关玉娟[1], 杨可立[1]

关键词：肝炎病毒, 乙型, 肝炎, 乙型, 慢陛, 癌, 肝细胞, 程序性凋亡因子1, Hepatitis B virus, Hepatitis B, chronic, Carcinoma, hepatocellular, Programmed death- 1

中文摘要：

目的研究慢性HBV感染者程序性死亡因子1（PD-1）基因的拷贝数和mRNA表达水平，以及在不同临床表现患者间的差异。方法实时荧光定量PCR法检测健康对照者27例（对照组）、慢性HBV携带者31例（携带组）、慢性重型肝炎患者19例（重肝组）、乙型肝炎相关性原发性肝癌患者29例（肝癌组）的外周血单个核细胞PD-1基因拷贝数及其mRNA的表达水平，分析各组间PD-1基因拷贝数及其mRNA水平表达的差异，以及PD-1基因拷贝数与其mRNA表达水平的关系。两组间率比较用Chi-square检验，多个样本间比较用秩和检验，多个样本间PD-1mRNA表达水平的两两比较用秩变换分析。结果106份样本中，PD-1基因拷贝数变异范围为0～3拷贝，单拷贝率在对照组、携带组、重肝组和肝癌组分别为37．o％、35．5％、26．3％和6．9％；2拷贝率分别为55．6％、58．毗、63．20／0和82．8％；3拷贝率分别3．7％、6．5％、10．5％、10．3％。将2～3拷贝合并，称为多倍体，多倍体率在对照组、携带组、重肝组和肝癌组分别为59．3％、64．5％、73．7％、93．1％，4组间PD-1基因多倍体率比较，x^2=9.583，P〈0.05，差异有统计学意义。与肝癌组比较，对照组和携带组多倍体率更低，X^2值分别为8．985、7．215，P值均〈0．05，差异有统计学意义。对照组、携带组、重肝组、肝癌组PD-1平均基因拷贝数分别为1．59士0．63、1．70±0．52、1．84±0．60、2．00±0．37。对照组、携带组、重肝组、肝癌组PD-1mRNA表达水平中位数分别为0．00254、0．00272、0．00255、0．00133。携带组、重肝组、肝癌组PD-1基因拷贝均数呈逐步上升趋势，而PD-1mRNA表达则呈下降趋势。3组慢性HBV感染者中，肝癌组、重肝组、携带组2个拷贝数个体的PD-1mRNA表达所对应的平均秩分别为19．59、32．57、33．22，肝癌组平均秩低于重肝组和携带组，F=5．395，P?

英文摘要：

Objective To study the copy numbers and mRNA expression levels of the Programmed Death-1 gene in chronic hepatitis B patients and to analyze the differences of the copy numbers and mRNA expression levels of the gene in patients with different clinical outcomes. Methods Real time PCR was adopted to detect the PD-1 gene copy numbers and their mRNA expressions in peripheral blood mononuclear cells （PBMCs） from 27 samples from healthy donors in Control group, 31 samples from chronic asymptomatic HBV carriers （ASC, n=31）, 19 samples from chronic severe hepatitis B patients （CSH, n=19） and 29 samples from Primary hepatitis B Virus-related hepatocarcinoma （PHC, n=29）. The differences and relationship of copy numbers and their mRNA expression levels among those groups were compared and analyzed by adopting Chi-square test and Rank sum test. Results PD-1 gene copy number deviated from 0 copy to 3 copies among all the 106 samples. In control group, ASC group, CSH group and PHC group, the percentages of cases of haploid （single） were 37.0%, 35.5%, 26.3% and 6.9%, respectively, the percentages of cases of diploid （double） were 55.5%, 58.0%, 63.2% and 82.8%, respectively, and the percentages of cases of triploid （triple） were 3.7%, 6.5%, 10.5% and 10.3%, respectively. The percentage of cases of polyploid （diploid and triploid） in control goup, ASC group, CSH group and PHC group were 59.3%, 64.5%, 73.7% and 93.1%, respectively. The different distribution of PD-1 gene copy number of polyploid was significant in total samples （x^2 = 9.583, P 〈 0.05）. Compared with Control Group and ASC group, the percentage of cases ofpolyploid in PHC group was lower with the x^2 equals to 8.985 and 7.215 respectively and both withP 〈 0.05. The difference between the two groups was statistically significant. The mean PD-1 gene copy numbers for these four groups were 1.59 ± 0.63, 1.70 ± 0.52, 1.84 ±0.60 and 2.00 ± 0.37 while the median were 0.002 54, 0.002 72, 0.002 55 and 0.001 33 respectively. Except

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