中文摘要：

根据BLAST获得的表达序列标签设计引物，从辣椒中分离并鉴定了一个新型转录因子，命名为CaRIN。与已知MADS—box蛋白对比表明，CaRIN分别与番茄LeMADS－RIN和矮牵牛FBP4有85％和77％的同源性，并且具有典型的MADS—box类转录因子的特征区域。采用半定量RT－PCR进行特异性表达分析，结果表明CaRIN在破色期和红熟期辣椒果实中大量表达，而在绿熟期辣椒果实和其他辣椒器官中几乎不表达；同时进行了DNA结合位点和系统发生等生物信息学分析，这些结果都表明CaRIN可能参与辣椒果实成熟的调控。

英文摘要：

Specific primers were designed based on the EST sequences which were obtained according to BLAST. A novel transcription factor CaRIN was isolated and characterized from the chili pepper （Capsicum annuum L. cv. Bukang）. Compared with known MADS-box proteins, CaRIN had 85% and 77% sequence homology with LeMADS-RIN from tomato and FBP4 from petunia, respectively, and contained typical structural characteristics of MADS-box transcription factors. Analysis of tissue-specific expression of CaRIN was performed by semi-quantitative RT-PCR. The result showed that CaRIN mRNA was existing mainly in breaker and red ripening fruits, but hardly detectable in mature green fruit and other organs of pepper. Bioinformatic analysises, such as DNA-binding residues and phylogenetic analysis, suggested that CaRIN were involved in regulating pepper fruit ripening.