中文摘要：

背景：研究表明,丝胶蛋白可以增加哺乳动物细胞的增殖,促进伤口愈合,具有良好的抗菌、抗氧化、抗癌的活性,同时具有良好的生物相容性和可降解性,是一种理想的组织工程材料。然而目前对丝胶蛋白能否引起炎症响应仍存在争论。目的：评价聚己内酯/丝胶蛋白静电混纺膜的炎症响应。方法：将不同混合比例的聚己内酯/丝胶蛋白纳米纤维薄膜体内埋植入大鼠背部肌肉,4周后取出进行组织学观察,通过苏木精-伊红染色和巨噬细胞抗体CD68免疫组织化学染色评价其炎症响应。结果与结论：聚己内酯/丝胶蛋白7∶3组混纺膜炎症反应与丝素蛋白膜类似,小于聚己内酯/丝胶蛋白6∶4组、聚己内酯/丝胶蛋白5∶5组和聚己内酯组;聚己内酯/丝胶蛋白6∶4组与聚己内酯/丝胶蛋白5∶5组与聚己内酯组类似。提示聚己内酯/丝胶蛋白静电混纺膜不会引起显著的炎症响应,少量添加丝胶蛋白可以降低聚己内酯的炎症反应,随着丝胶蛋白含量的增加,炎症反应呈上升趋势。

英文摘要：

BACKGROUND： Studies have shown that silk sericin （SS） can accelerate the proliferation of mammalian cells and promote wound healing. SS has good activities for antibacteria, antioxidation and anticancer. SS also has a good biocompatibility and biodegradability. It is an ideal material for tissue engineering, however, there is still a controversy of whether SS causes inflammatory response so far. OBJECTIVE： To evaluate the inflammatory response of electrospun polycaprolactone （PCL）/SS membranes. METODS： Materials with different PCL/SS ratios were implanted into the back muscles of rats and were taken out after 4 weeks. The inflammatory response was evaluated by hematoxylin eosin staining and macrophage antibody CD68 immunohistochemistry. RESULTS AND CONCLUSION： The inflammatory response of the 7：3 group of PCL/SS membranes is similar to that of silk fibroin membrane and was less than those of the 6：4, 5：5 and PCL groups. The 6：4 and 5：5 groups have similar inflammatory response with PCL group. Electrospun PCL/SS membranes can not cause significant inflammatory response. A little amount of SS can decrease the inflammatory response of PCL membrane. However, the inflammatory response of PCL/SS membrane can increase as the ratio of SS to PCL increased.