解决珍贵用材树种福建青冈种子易萌和人工育苗困难等问题,以成熟健康未发芽的福建青冈种子为试验材料,以不施外源激素为对照处理,分别利用5个浓度(0、10、20、30、40 mmol·L^-1)的赤霉素和脱落酸对种子进行处理,并于激素处理后3、5、7、9和13 d测定种子胚根和子叶内抗氧化酶系统的活性。结果表明:2种外源激素处理对福建青冈种子萌发率的影响存在明显差异。随赤霉素浓度增加,种子萌发率呈逐渐增高的趋势,但处理间差异不显著(P〉0.05)。低浓度(10~20 mmol·L^-1)脱落酸处理可明显提高种子萌发率,高浓度(30~40 mmol·L^-1)则呈抑制作用。随着外源激素处理时间的延长,胚根与子叶过氧化物酶(POD)、超氧化物歧化酶(SOD)活性均呈先增强后不规则的变化趋势。在不同赤霉素浓度处理下,胚根过氧化氢酶(CAT)活性随时间的延长逐渐增强,子叶CAT活性呈倒“N”型变化趋势。施加40 mmol·L^-1脱落酸处理后,福建青冈种子胚根与子叶多酚氧化酶(PPO)活性明显低于对照处理,显著抑制种子萌发,可有效解决种子易萌性问题。
To solve the problems of quick seed germinating and artificial seedling difficulty of Cyclobalanopsis chungii, a precious timber species, the healthy, mature and nongerminating seeds were selected as experimental material. The antioxidant enzyme system activity in radicle and co-tyledon of the seeds was determined in 3, 5, 7, 9 and 13 days after treating with five different concentrations (0, 10, 20, 30, 40 mmol·L^-1) of two kinds of hormone (gibberellin acid and abscisic acid). The results showed that there were significant differences in germination rate and antioxidant enzyme activity among different hormone treatments. With the increase of the gibberellin acid concentration, the germination rate of seeds increased markedly, but there was no significant difference among the treatments. The abscisic acid with low concentration (10-20 mmol·L^-1) promoted the germination rate significantly, but not with high concentration (30-40 mmol·L^-1). With extension of the processing time of exogenous hormones, POD and SOD activities in the radicle and cotyledon showed a tendency of increase firstly then exhibited irregular changes. Under different concentrations of gibberellin acid, CAT activity in the radicle increased gradually with prolonging time, whereas CAT activity in the cotyledon demonstrated an inverted “N” pattern. Applying with 40 mmol·L^-1 abscisic acid could solve the problem of quick germinating by inhibiting PPO activity in the radicle and cotyledon.