中文摘要：

为优化茶树ISSR分子标记反应体系,对影响茶树ISSR反应较大的Mg2＋浓度、dNTP浓度、模板DNA浓度、TaqDNA聚合酶浓度、引物浓度5个因素在4水平上进行优化试验,建立了适合于茶树ISSR-PCR（Inter Simple Sequence Repeats-Polymerase Chain Reaction）的最佳体系：20μL反应体系中,TaqDNA聚合酶0.75U/μL,10×buffer（含Mg2＋）2.0mmol·L-1,模板DNA20ng,dNTP0.1mmol·L-1,引物0.3μmol·L-1。反应程序为：94℃预变性5min,94℃变性1min,50～60℃退火40s,72℃延伸90s,34次循环,72℃延伸7min,4℃保存。

英文摘要：

In order to optimizing ISSR reaction system in tea, the concentrations of Mg2＋, dNTP, DNA template, Taq DNA polymerase and primer DNA were optimized at four levels by orthogonal design, effects of the five factors on ISSR amplification in tea plants were rather significant.The optimized ISSR-PCR system for tea plants was established as follows： 0.75 U/μL Taq DNA polymerase, 2.0 mmol·L-1 Mg2＋, 20 ng DNA template, 0.1 mmol·L-1 dNTP and 0.3 μmol·L-1 primer were contained in 20 μL reaction system, and the reaction procedure was shown as below： 94 ℃ for 5 min, followed by 34 cycles of 94 ℃ for 1 min, annealing for 40 s at 50-60 ℃, and extending for 90 s at 72 ℃, and was terminated for 7 min at 72 ℃, then stored at 4 ℃.