中文摘要：

采用SEM和氨基酸自动分析仪对横纹金蛛（Argiope bruennichi）卵袋的超微结构与氨基酸组成进行了观察研究。结果表明,横纹金蛛卵袋呈封闭的葫形,是由多种丝腺纺出的微米与纳米级的丝纤维形成的多个覆盖层构成,具有多层复杂结构,从外向内分别为：框架、卵袋覆盖帽、外覆盖层和内覆盖层、卵块覆盖帽、卵块包裹层,其中外覆盖层与卵袋覆盖帽内侧面的丝较外侧面的丝排列规则均匀。卵袋框丝与典型的拖牵丝（大壶状腺丝）氨基酸组成相似,甘氨酸和丙氨酸的含量最丰富,分别约占37%和25%,其次是谷氨酸、脯氨酸和丝氨酸,分别约占10%、7%和5%;其余卵袋丝纤维的氨基酸组成与典型的柱状腺丝相似。与卵袋框丝相比,卵袋覆盖层的丝氨酸含量显著增加,约占22%,同时甘氨酸与脯氨酸的含量显著减少,分别约占7%与2%,并含有更多的极性和大侧链氨基酸。本文根据氨基酸组成与扫描电镜的结果综合分析了不同直径丝纤维的丝腺来源。

英文摘要：

The structures and fibrous composition of egg case of the spider（Argiope bruennichi） were examined using SEM as well as amino acid analysis.The egg case is composed of multiple layers of silk,including the frame,outer cover,cover cap,inner cover,egg cover cap and egg wrapping cover,and formed by different colored silk with diameters between hundreds of nanometers and several micrometers.The amino acid composition was similar in the frame silk and typical dragline silk,glycine（about 37%） and alanine（about 25%） are the most abundant amino acids present,and glutamic acid（about 10%）,prone（about 7%） and serine（about 5%） as the next most abundant.The amino acid composition of cover silk was similar with typical cylindrical gland silk.In contrast to the frame silk,all cover silk of egg case contained a significant proportion of the serine（about 22%） and less glycine（about 7%） and prone（about 2%）,and contained more polar amino and large side acids.The original gland that produce silk fibers with different diameters was analyzed based on the results of SEM and amino acid composition studies.