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Resonance Scattering Effect of Dopamine Product Particle and Its Application to Polyphenoloxidase Activity Assay
  • ISSN号：1001-604X
  • 期刊名称：《中国化学：英文版》
  • 时间：0
  • 分类：O614.122[理学—无机化学;理学—化学] TS255.54[轻工技术与工程—农产品加工及贮藏工程;轻工技术与工程—食品科学与工程]
  • 作者机构：[1]Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China
  • 相关基金：Project supported by the National Natural Science Foundation of China （Nos. 20865002, 20965002, 21075023）, the Natural Science Foundation ot Guangxi （Nos. 0991021 z, 0832260） and the Research Funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection （Guangxi Normal University）, Ministry of Education.
作者： Jiang, Caina Ling, Shaoming Liang, Aihui Ouyang, Huixiang Wang, Pengfei Jiang, Zhiliang[1]
关键词： 多酚氧化酶活性, 共振散射光谱, 酶活性测定, 散射效应, 应用, 多巴胺, PPO活性, 粒子, polyphenoloxidase, dopamine, organic particle, resonance scattering spectral assay
中文摘要：

在 pH 7.2 Na2HPO4-NaH2PO4 缓冲区答案， polyphenoloxidase (PPO ) 能催化多巴胺(DA ) 的氧化在黑暗红的颜色形成聚合物粒子，它展出了散布的强壮的回声(RS ) 在 780 nm 的山峰。处于选择条件，当 PPO 活动增加了，在 780 nm 的 RS 紧张线性地增加了。增加的 RS 紧张(I780 nm ) 对在 0.106.0 U 的范围的 PPO 活动线性

英文摘要：

In pH 7.2 Na2HPO4-NaH2PO4 buffer solution, polyphenoloxidase （PPO） could catalyze the oxidization of do- pamine （DA） to form polymer particles in dark-red color, which exhibited a strong resonance scattering （RS） peak at 780 nm. In the chosen conditions, as the PPO activity increased, the RS intensity at 780 nm increased linearly. The increased RS intensity （△I780nm） was linear to the PPO activity in the range of 0.10--6.0 U·mL^- 1, with a regression equation of △I780 nm = 96.6C＋ 15.1, a relative coefficient of 0.9987 and a detection limit of 0.06 U·mL^-1 PPO. The proposed method was applied to detecting PPO activity in potato sample with satisfactory results.

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期刊信息
  • 《中国化学：英文版》
  • 主管单位:
  • 主办单位:中国化学会
  • 主编：
  • 地址：上海市枫林路354号中科院上海有机化学研究所
  • 邮编：200032
  • 邮箱：
  • 电话：021-54925243
  • 国际标准刊号：ISSN：1001-604X
  • 国内统一刊号：ISSN：31-1547/O6
  • 邮发代号:4-646
  • 获奖情况:
  • 中国期刊方阵“双高”期刊
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,荷兰文摘与引文数据库,美国科学引文索引（扩展库）,日本日本科学技术振兴机构数据库,英国英国皇家化学学会文摘
  • 被引量:175