对采自钱塘江口沿岸滩涂的大鳍弹涂鱼Periophthalmus magnuspinnatus的个体繁殖力进行了估算,采用半干法获取了受精卵,并在显微镜下进行了胚胎发育观察,盐度梯度实验分析了胚胎发育的盐度耐受性。结果表明,大鳍弹涂鱼的个体绝对繁殖力(F)为2249—3472(2746±230)粒;个体相对繁殖力FL为32—44(35±2)粒/mm,FW为643—1320(698±71)粒/g。雌性注射LHRH-A30.2μg和HCG20 IU的剂量、雄性注射HCG 20 IU的剂量,均能促使健壮个体的性腺成熟。大鳍弹涂鱼的胚胎发育过程可划分为30个发育期,在水温27℃、盐度10‰、pH(7.8±0.3)的条件下,经历158h46min才孵出仔鱼,所需的积温达4286.70 h.℃。初孵仔鱼器官发育完善,已形成胸鳍、鳔、肾脏和膀胱等器官,眼睛有大量黑色素沉积,心率达283次/min。在5‰、10‰和15‰的盐度条件下,胚胎均能正常孵化,但胚胎发育的时间和发育积温随盐度的降低而减小。在20‰、25‰、30‰和35‰的盐度条件下,胚胎在发育过程中死亡。
Periophthalmus magnuspinnatus inhabit in China which was previously mistaken as Periophthalmus modestus and recently reported from the coasts of Bohai Sea,Yellow Sea,East China Sea,and South China Sea.For the taxonomically new P.magnuspinnatus,there is only one study on the skin structure and investigating the life history Lifestyle of Korean mudskipper.This paper was studied the fecundity and effects of salinity on embryonic development of the P.magnuspinnatus in Qiantang River estuary.The fertilized eggs were observed through a semi-dry artificial fertilization method,which were cultured and analysed in constant temperature of sea water with weak aeration in different salinities.The embryonic development of mudskipper was characterized by continual viviperception.The results indicated that the individual absolute fecundity(F) ranged from 2249 to 3472 eggs,the average was 2746 eggs,and the standard error of mean was 230.The individual relative fecundity(FL) ranged from 32 to 44 eggs per millimeter,the average was 35 eggs,and the standard error of mean was 2.The individual relative fecundity(FW) ranged from 643 to 1320 eggs per gram,the average was 698 eggs,and the standard error of mean was 71.From the injection of 0.2 μg of LHRH-A3(luteinizing hormone releasing hormone-analogue) then after 24 hours injection of 20 IU of HCG(Human Chorionic Gonadotropin) into female fish and 20 IU of HCG into male fish,each male fish received one injection at the same time as female fish received the second injection.Most of experimental fish was able to develop to mature.With the water temperature of 27 ℃,salinity of 10‰,and pH of 7.8±0.3,the embryonic development required 158 hours and 46 minutes,while the accumulated temperature was up to 4286.70 h.℃.The development time from heart beating to hatch larva was relatively longer which need 127 hours and 52 minutes under 27 ℃ and 10‰ salinity condition,accounting for 80.5% of the total embryonic development time.The development time from air bladde