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PCR-DGGE研究沸石植生混凝土微生物群落结构
  • ISSN号:1000-6923
  • 期刊名称:《中国环境科学》
  • 时间:0
  • 分类:X522[环境科学与工程—环境工程]
  • 作者机构:[1]湖南农业大学环境工程系,湖南长沙410128, [2]环境保护部华南环境科学研究所,广东广州510655, [3]暨南大学环境工程系,广东广州510632, [4]南华大学市政工程系,湖南衡阳412001
  • 相关基金:国家自然科学基金项目(50908095);广东省自然科学基金(9451065501002521);国家公益性科研院所资助项目(ZX-200712-29) 致谢:本实验样品的预处理过程中得到了东江水环境研究中心(惠州站)所有工作人员的支持,在论文修改过程中,得到了阎佳博士的悉心指点,在此表示感谢.
中文摘要:

采用PCR-DGGE技术研究了滨水区和非滨水区沸石植生混凝土内部微生物群落结构,结果表明:沸石植生混凝土内部微生物多样性指数和物种丰度值均很高,滨水区和非滨水区植生混凝土内部微生物多样性指数与物种丰度总体持平,但是各部分微生物丰度差异明显.滨水区微生物丰度值根部5~10cm〉根部10-15cm〉沸石表面生物膜.非滨水区微生物丰度沸石表面生物膜〉根部5~10cm〉根部10-15cm.基因测序结果和系统发育树分析可知.滨水区和非滨水区以及沸石混凝土内各部分之间优势菌种各不相同,在滨水区,沸石表面微生物膜中优势菌种为丙酸杆菌、都柏林克罗诺杆菌和葡萄球菌属,根部5~10cm中优势菌种为黄杆菌和沙门氏菌属,根部10~15cm中优势菌种为慢生根瘤菌属;非滨水区,沸石表面微生物膜中优势菌种为芽孢杆菌和红假单胞菌属,根部5-10cm中优势菌种为沙门氏菌、微球菌亚目的Agromyces和酸杆菌属,根部10~15cm中优势菌种为沙门氏菌属.

英文摘要:

The microbial community composition in zeolite-vegetation-concrete which was grown in waterfront and embankment was investigated in this study with PCR-DGGE technology. The results showed that: Shannon diversity index and species abundance value of microbial community in the system were high, and the community composition in each section of the zeolite-vegetation-concret were very different depending on the depth. The order of species abundance value growed in waterfront of zeolite-vegetation-concrete was as followed (from high to low): rhizosphere soil (depth:5-10cm)〉 rhizosphere soil (depth:10-15cm)〉zeolite surface biofilm, however, the order of species abundance value growed in embankment was very different from the order of waterfront section, which was zeolite surface biofilm〉 rhizosphere soil (depth:5-10cm)〉rhizosphere soil (depth: 10-15cm). Phylogenetic analysis revealed that, the pre-dominant species in zeolite-vegetation-concrete were different in each section. In waterfront section, the pre-dominant species in zeolite surface biofilm were Propionibacterium sp., Cronobacter dublinensis and Staphylococcus epidermidis, then Flavobacterium sp. and Salmonella sp. were the major microbe in rhizosphere soil with depth of 5-10cm, and Bradyrhizobium sp. was found to be the pre-dominant bacteria in rhizosphere soil with depth of 10-15cm. In embankment section, the pre-dominant microbe in zeolite surface biofilm were Paenibacillus sp. and Rhodopseudomonas sp., Salmonella sp., Agromyces sp. and Acidobacteria sp. were the major microbe group in rhizosphere soil with depth of 5-10cm, and Salmonella sp. was found to be the pre-dominant bacteria in rhizosphere soil with depth of 10-15cm.

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期刊信息
  • 《中国环境科学》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国环境科学学会
  • 主编:王文兴
  • 地址:北京市海淀区红联南村54号
  • 邮编:100082
  • 邮箱:zghjkx1981@126.com
  • 电话:010-62215145
  • 国际标准刊号:ISSN:1000-6923
  • 国内统一刊号:ISSN:11-2201/X
  • 邮发代号:2-572
  • 获奖情况:
  • 国家期刊提名奖,国家“双效”期刊,第三届中国科协优秀科技期刊一等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,荷兰文摘与引文数据库,美国工程索引,美国剑桥科学文摘,英国科学文摘数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:47702