中文摘要：

目的：进一步明确二相伤寒沙门菌鞭毛抗原表达调节机制，系统分析伤寒沙门菌fljA样基因功能，构建含倒样基因的表达载体并验证其表达。方法：根据倒样基因两端序列设计引物，以z66抗原阳性伤寒沙门菌GIFU10007基因组为模板，通过PCR获得该基因，与表达载体pET22b连接后，重组体转化至大肠杆菌JM109中诱导培养，并通过RT-PCR观察傅4样基因表达情况。结果：基因克隆和序列分析结果显示伤寒沙门菌庳4样基因被成功导入载体pET22b，RT-PCR结果提示大肠杆菌JM109可利用此重组载体进行西4样基因表达。结论：成功获得能在大肠埃希菌中表达伤寒沙门菌西4样基因的载体。

英文摘要：

Objective： To reveal the function of a fljA-like gene of Salmonella enterica serovar Typhi, a vector expressing thefljA-like gene was constructed. Methods： Specific primers were designed as the previous sequence to amplify the fljA-like gene from the chromosome DNA of S. enterica serovar Typhi GIFU 10007, a wild z66 antigen positive strain. The amplicon was inserted into the vector pET22b, which was then transferred to E. coli JM109. The expression of thefljA-like gene was also investigated by RT-PCR with total RNA of the host cell. Results： Sequencing of the vector provided that the fljA-like gene was inserted into the vector and RT-PCR showed the transcriptional expression of the gene in the host cell. Conclusion： A vector to express the fljA-like gene of S. enterica serovar Typhi in E. coli was acquired successfully.