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中文摘要：
轮藻是一种广泛分布且具有一定经济价值的水生植物，但其人工培养鲜见报道。在M-11培养基的基础上，调整N／P为16：1、40：1和80：1，Fe2＋离子浓度为0.3、0．6、0．8mol／L等进行轮藻培养，通过称重法研究适合轮藻人工培养的条件。结果显示，轮藻培养的最适N／P为40：1，最适Fe2＋离子浓度为0．3mol／L。进而得出一种适宜轮藻培养的培养基配方（mg／L）：NaNO3 250，KH2PO4 10，MgCl2·7H2O 29，CaCl2·5H2040，Na2CO3 20，Na2EDTA·2H2O1，EDTA-NaFe2．25。
英文摘要：
Charophytes are one of most structurally complex green algae with a worldwide distribution and economic value, while no research has been conducted on culture conditions. In this study, on the basis of the M-11 medium, the N/P and Fe2＋ concentration had been adjusted 16：1, 40：1, 80：1 and 0.3 tool/L, 0.6 mol/L, 0.8 mol/L separately. The culture conditions of Charaphytes were studied by weighing method. The conclusion showed that the best N/P was 40：1 and the suitable Fe2＋ concentration was 0.3 mol/L. So the optimized culture of Charaphytes was NaNO3 250 mg/L, KH2PO4 10 mg/L, MgCl2·7H2O 29 mg/L, CaCl2·5H2O 40 mg/L, Na2CO3 20 mg/L, Na2EDTA. 2H2O 1 rag/L, EDTA-NaFe 2.25 mg/L.
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