中文摘要：

目的探讨Tmubl蛋白在肝再生过程中的作用及其在肝再生过程中对Securin蛋白的影响。方法54只大鼠随机分为3组即TmublRNAi慢病毒组、空载慢病毒组及正常对照组，其中TmublRNAi慢病毒组、空载慢病毒组注射相应病毒颗粒（3×10’TU），对照组未行注射，2d后行肝部分切除术（partialhepatectomy，PH），每组分为6个亚组：PH术后0、2、6、12、24、48h，每亚组3只大鼠，术后提取原代肝细胞、留取肝脏组织标本，利用Real—timePCR和Westernblot检测慢病毒干扰效果及Tmubl沉默后对Securin蛋白的影响。M1Tr实验、流式细胞仪检测原代肝细胞的增殖情况。结果Real．timePCR和Westernblot表明实验组TmublmRNA和蛋白被有效抑制；Tmubl沉默后securinmRNA表达无明显变化，而G2／M期securin蛋白量明显减少。MTT实验表明Tmubl沉默可明显上调PH术后6～24h肝细胞的增殖速率；流式细胞分析结果显示实验组处于G2／M期的肝细胞比例明显增高。结论Tmubl蛋白在肝再生过程中对肝细胞增殖进程发挥负向调控作用，而该作用可能与其在蛋白质水平影响了Securin的表达密切相关。

英文摘要：

Objective To investigate the role of transmembrane and ubiquitin-like domain-containing protein 1 （ Tmubl ） protein in the liver regeneration and its impact on the expression of securin in the process. Methods A total of 54 adult male SD rats were randomly divided into Tmubl RNAi lentivirns group, blank lenitiviral vector group, and control, and they received an injection of lentivirns （3 x 107TU） or PBS through ileocaecal vein. In 2 d later, partial hepatectomy （PH） were carried out in all rats. Then the liver tissue was collected in 0, 2, 6, 12, 24 and 48 h after model establishment to extract primary hepatocytes and preserve liver tissue. Effect of Tmubl RNAi lentivirus interference on rat primary hepatocytes was detected by real-time PCR and Western blotting, respectively. Effect of Tmubl RNAi lentivirns on the growth and cell cycle in rat primary hepatocytes was detected by MT1~ assay and flow cytometry, respectively. Results Real-time PCR and Western blotting showed that the expression of Tmubl at mRNA and protein levels was significantly inhibited, and the expression of securin at mRNA level had no change, but the expression of securin at protein level was significantly down-regulated. MTT assay showed that Tmubl gene silencing significantly improved the proliferation in liver cells in 6 to 24 h after PH. Flow cytometry displayed that the liver cells were arrested in G2/M phase. Conclusion Tmubl protein plays a negative role in the process of liver cells＇ proliferation, which may be closely related to its effect on the expression of securin at protein level.