中文摘要：

探讨复方柴归方超临界CO_2萃取组分抗抑郁作用机制。利用TCMSP、HIT、Pharm GKB和Gene Cards等生物信息学手段对药物、疾病及其共有靶点进行预测;复方柴归方超临界CO_2萃取组分干预慢性温和不可预知应激抑郁模型大鼠后收集血清和海马组织,采用酶联免疫吸附（enzyme-linked immunosorbent assay,ELISA）法对预测的潜在靶点进行定量分析。预测结果显示,组分中已鉴定的22个化学成分共有78个潜在药物靶点;抑郁症发生及发展相关的疾病靶点共有177个;二者共有靶点14个,分别是神经递质转运体、代谢酶和受体;下丘脑-垂体-肾上腺/甲状腺/性腺轴分泌激素,免疫相关因子等。ELISA结果表明,复方柴归方超临界CO_2萃取组分能够显著调节在模型组大鼠海马组织中明显降低的磷酸环磷腺苷效应原件结合蛋白水平和在血清中升高的皮质酮、白介素6水平。本研究利用网络药理学的思路对复方柴归方超临界CO_2萃取组分的抗抑郁机制进行了探讨,为中药复方有效组分的研究提供了新思路和新方法。

英文摘要：

The study was designed to clarify the antidepressant mechanism of supercritical CO_2 extract from Compound Chaigui Fang. We used TCMSP, HIT, Pharm GKB and Gene Cards bioinformatics software to predict and analyze the drug/disease targets and their common targets of compounds in the supercritical CO_2 extract of Compound Chaigui Fang for depression. Chronic unpredictable mild stress（CUMS） was used to induce depressed model in rats. Hippocampus and serum were collected after supercritical CO_2 extract treatment to detect the potential antidepressant targets with enzyme-linked immunosorbent assay（ELISA）. The results predicate that there are 22 chemical compounds and 78 potential therapeutic drug targets for depression. There are 177 disease markers for depression, and 14 common targets for drug intervention of depression, which includes the neurotransmitter transports/metabolic enzyme/receptors, hormone of hypothalamus-pituitary-adrenal/ thyroid/gonad axis, immune-associated factor, etc. ELISA results suggest that depression is associated with the low level of phosphate c AMP responsive element-binding protein in hippocampus and the high levels of corticosterone and interleukin 6 in serum with CUMS rat. Those were restored to normal levels by supercritical CO_2 extract of Compound Chaigui Fang. The study provides an antidepressant mechanism of supercritical CO_2 extract of Compound Chaigui Fang based on the network pharmacology, and a new strategy in the study of the effective extracts of compound Chinese traditional medicine.