为了解高山红景天离体培养中的光温条件,探讨了几种光温梯度对高山红景天愈伤组织诱导及生长的影响。结果表明:高山红景天愈伤组织诱导效果较好的光照长度为14h/d、光照强度为较低的1 000lx;高山红景天在较低的温度(20~24℃)条件下愈伤组织诱导效果较好,而在较高的温度不利于愈伤组织诱导,愈伤组织生长有利的温度为24℃。
In order to understand light and temperature in the Rhodiola vitro culture,the effect of several light and temperature gradient on the induction of Rhodiola callus and growth were studied.The results showed that the light length of better induction effect of Rhodiola callus was 14 h/d,light intensity was 1 000 lx which was lower;The better induction effect of Rhodiola callus in the lower temperature(20~24℃),the higher temperature go ahainst induction of callus,the favorable temperature of callus growth was 24℃.