中文摘要：

目的探讨雌激素（17β-estradiol）对大鼠骨髓基质干细胞分化的成骨细胞上皮钠离子通道（ENaC）表达和功能的影响。方法采用大鼠骨髓基质干细胞来源的成骨细胞，通过CCK-8试剂盒和AKP试剂盒分别测定雌激素对细胞增殖和分化的影响，通过半定量PCR方法测定雌激素对成骨细胞ENaC和成骨相关基因表达的影响。结果雌激素能促进初级成骨细胞增殖，且较高浓度时（1×10 -7，1×10 -5mol·L-1）促增殖作用显著（与对照组相比，P〈0．05）；联用阿米洛利处理后各组细胞增殖速度均有所降低，其中高浓度E2组显著降低（与E2单独处理纽相比，P〈0．05）；雌激素能提高成骨细胞上a-ENaC、γ-ENaC基因的表达水平，且增加趋势与成骨相关基因（Coll—Ia、OC、ALP、ON）mRNA表达的增加趋势一致。结论雌激素促进骨形成的同时也增加ENaCmRNA表达，而阿米洛利（ENaC抑制剂）能阻断雌激素的促成骨作用，说明ENaC可能参与成骨细胞成骨，提示成骨细胞可能存在一个与ENaC相关的调节新途径，为骨代谢的研究提供了一个新思路。

英文摘要：

OBJECTIVE To study the regulation effects of 17/3-estradiol on the expression and function of the rat osteoblast （de- rived from bone marrow stromal cells） epithelial sodium channel（ENaC）. METHODS Cell proliferation and differentiation were e- valuated by WST-8 assay kit and AKP assay kit respectively. Regulation effects on mRNA expression of ENaC and osteogenesis gene were investigated by semi-quantitative PCR. RESULTS 17β-estradiol promoted the osteoblasts proliferation, the difference among administration group（ E2 ： 1 × 10 -7 and 1 × 10 -5 mol · L -1 ） and control group had statistical significance（ P 〈 0. 05 ）, and the promoting effect was inhibited by amiloride （P 〈 0.05, compared with Amiloride untreated cells） , an inhibitor of ENaC. 17β-estradiol also induced the a-ENaC, γ-ENaC and osteogenesis gene（ Coll-Ia, OC, ALP and ON） expression, and same tendency was seen. CONCLU- SION 17β-estradiol stimulated proliferation and ENaC mRNA expression in rat osteoblast, and the promoting effect of 17β-estradiol was blocked by amiloridc. ENaC participates in osteoblast bone formation. The regulation of ENaC channel expression and function may provide a new clue for research on bone metabolism.