中文摘要：

以5-碘-2’-脱氧尿嘧啶为起始原料，5（6）．羧基荧光素为荧光标记物，经6步反应合成了5（6）．羧基荧光素标记的2＇-脱氧尿苷，并利用高效液相色谱（HPLC），^1H NMR和^13C NMR对目标化合物的纯度和结构进行了表征．

英文摘要：

2＇-Deoxyuridine labeled with 5（6）-carboxylfluorescein was synthesized in six steps from 5-iodo-2＇-dexoyuridine. Their structures were analyzed and characterized using HPLC, ^1H NMR and ^13C NMR spectra.