中文摘要：

首先利用壳聚糖（CHIT）和戊二醛将乙酰胆碱酯酶（ACh E）固定到金盘载体表面,然后利用固定的乙酰胆碱酯酶催化乙酰硫代胆碱（ATCl）水解,使生成的硫代胆碱（TCl）组装到一支裸金电极表面。以三联吡啶钌（Ru（bpy）32＋）作为发光试剂,以自组装在金电极表面的硫代胆碱作共反应物,在1 500 m V获得了灵敏的电化学发光信号。基于甲基对硫磷对固定化的ACh E抑制的原理建立了电化学发光法测定甲基对硫磷的方法。在优化的实验条件下,酶的抑制率与甲基对硫磷浓度的对数在4.6×10^-6-4.0×10^-3mol/L浓度范围成正比关系,检测限为2.3×10^-6mol/L。

英文摘要：

Chitosan and glutaraldehyde were used to immobilize acetylcholinesterase （ACHE） onto the surface of gold disc carrier. Then acetylthiocholine （ATCl） hydrolysis was catalyzed by the immobilized acetylcholinesterase to produce thiocholine （TCl） which can be accumulated onto the surface of a gold disc electrode by self-assembly. A sensitive electrochemiluminescence signal was observed at about 1 500 mV with thiocholine as coreactant in the presence of tris（2,2 ＂ -bipyridyl）ruthenium（ Ⅱ ） complex. An electrochemiluminescence method was developed to detect methyl parathion based on the principle of acetylcholinesterase inhibition by methyl parathion. The inhibition rate of the enzyme is linearly increased with the increase of the logarithm values of methyl parathion concentration over the range of 4.6×10^-6-4.0×10^-3 mol/L. The detection limit is 2.3×10^-6 mol/L.