中文摘要：

本研究针对在绵羊IGFBP-3基因内含子4区新发现的1个SNP位点（C218T）,建立了PCR-RFLP的基因分型方法。利用该方法对353只中国美利奴羊（新疆军垦型）进行了多态性检测,结果显示,有CC（249bp）、CT（249/216/33bp）和TT（216/33bp）3种基因型,基因型频率分别为0.52、0.41和0.07;有C、T2种等位基因,等位基因频率分别为0.72、0.28。多重比较结果表明,不同基因型对中国美利奴部分羊毛生产性能有一定的影响,CC基因型个体的毛丛长度显著短于TT基因型个体（P〈0.05）,其毛囊密度显著高于CT和TT基因型个体（P〈0.05）。

英文摘要：

In this study, PCR-RFLP method was established according to a novel SNP site （C218T） within the intron 4 of sheep insulin-like growth factor binding proteins-3 （IGFBP-3） gene. Using this method, 353 Chinese Merino sheeps （Xinjiang Junken type） were tested and illustrated three unique binding genotypes of CC （249 bp）, CT （249/216/33 bp） and TT （216/33bp）. Frequencies of the genotype CC, CT, TT were 0.52, 0.41, 0.07, respectively, and frequencies of the allele C, T were 0.72 and 0.28, respectively. Different genotypes slightly affected several wool traits of Chinese Merino sheeps. Staple length （SL） in individuals of genotype CC was significantly shorter than that in individuals of genotype TT （P0.05）, follicle density in individuals of genotype CC was significantly higher than that in individuals of genotype CT and TT （P0.05）.