中文摘要：

探讨了家兔窦房结（Sino—Atrial Node，SAN）细胞的分离方法及其形态和起搏电流（FunnyCurrent。λ）的特征。通过打结定位法确定了带有“中心结区”的家兔SAN组织条，双酶法分离SAN细胞，利用全细胞穿孔膜片钳技术记录SAN细胞的^。结果显示。SAN组织为上宽下窄的枣核型，区域体积约为6．25mmx2．23mmx0．26mm，或6mmx2mmx0．1mm--6mmx2mmx3．3mm。SAN细胞成狭长梭形，无清晰横纹。细胞长（65．3±7．8）um，宽为（7．2±3．1）μm。在正常Tyrode液中，可恢复规则自律性搏动，频率为（187．3±5．6）次／mjn，典型SAN细胞数量占整个分离细胞的2％一5％。根据电压钳制方案，λ电流被引出，呈内向性斜坡状，随着钳制电压降低，起搏电流逐渐增大，并随钳制时间延长，倾斜度也增大。λ带有明显尾电流。也随着超级化激活电位降低。逐渐增大。根据二者电流大小拟合的电流一电压曲线。呈典型横向Y形。本法获得SAN细胞形态典型，状态和活性良好，能够记录具有特征性的／λ．可供心血管领域有关针对SAN细胞λ研究的技术借鉴。

英文摘要：

The isolation means and properties of morphology and pacemaker current λ in Sino-Atrial Node （SAN） cells of rabbits are investigated. In the present study, the SAN trip containing ＂the central nodal region＂ is located by tagging super vena cava with black fine tread as a mark in right atrium of rabbit. The beating pacemaker cell of the SAN is spontaneously isolated by two different enzymatic digestions. Hyperpolarization-activated pacemaker current is recorded by using whole-cell perforated patch technique in SAN cells. The results show that SAN tissue which include the ＂central nodal region＂ is shaped with superior wide and inferior narrow, and with an volume size that is approximately 6.25mmx2.23mm~0.26mm or 6mm~2mm~0.1mm--6mmx2mmx3.3mm. SAN cells are long thin spindle-shaped with faint cross-striations. They are measured with （65.3＋7.8）1xm in length and （7.2＋3.1）μm in width. In normal Tyrode＇s solution, the typical SAN cells which are 2%--5% in yielded all of them are recovered spontaneous beating with frequency of （187.3＋5.6） times/min. According to voltage clamp protocol, If is elicited as the inward ramp current, and becomes larger as soon as is activated more rapidly with the increasingly negative potential. The tail current of If has similar characteristics in same conditions. The current-voltage curve of λ and tail is appearing as a typical horizontal ＂Y＂ according to their current density and clamp potential. It could be identified that the isolated SAN cells have typical morphological properties and activity, therefore could be completely elicited inward ramp λ, the fact could contribute to the different investigations on λin SAN cells in cardiovascular areas.