中文摘要：

Marek 的疾病病毒(MDV ) 的完全的染色体紧张 GX0101，与加快的 LTR 序列综合，作为一个细菌的人工的染色体(用绳子拖的平底渡船) 在 Escherichia 关口 i 被克隆。用绳子拖的平底渡船向量序列被相应再结合介绍进 MDV 染色体的 US2 地点。包含 BAC 向量的病毒的 DNA 被用来转变 DH10B 的 Escherichia 关口 i 紧张。然后， recombinant 病毒被 recombinant 用绳子拖的平底渡船 DNA 的 transfection 成功地救进主要的鸡胚胎成纤维细胞(CEF ) 。这用绳子拖的平底渡船病毒的克隆被称为 bac-GX0101。当重新组成的病毒被接种进 1-day-old 鸟时，内脏的肿瘤能象 62 d 柱子感染一样早被检测。处于生长能力没有差别，到在 BAC 之间的鸟的致病力导出病毒和它的父母病毒。用绳子拖的平底渡船导出维持的病毒它的 oncogenicity 和抑制免疫力的效果。在结论， GX0101 紧张的完全的染色体成功地被克隆进用绳子拖的平底渡船，传染克隆被救。与强大的用绳子拖的平底渡船操作系统，传染克隆将为进一步在 MDV 的 GX0101 紧张理解插入的 REV-LTR 顺序的功能的角色提供一个有用工具。

英文摘要：

The complete genome of Marek＇s disease virus （MDV） strain GX0101, which was integrated with the LTR sequences of REV, was cloned in Escherichia coli as a bacterial artificial chromosome （BAC）. BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination. The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B. Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast （CEF）. This BAC viral clone was named bac-GX0101. When the reconstituted virus was inoculated into 1-day-old birds, visceral tumors could be detected as early as 62 d post infection. There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus. The BAC derived virus maintained its oncogenicity and immunosuppressive effects. In conclusion, the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued. With the powerful BAC manipulation system, the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV,