中文摘要：

目的：观察化痰消瘤方对lewis肺癌荷瘤小鼠瘤组织中P-gp、GSH表达的影响,探讨其抗多药耐药的作用机制及原理。方法：制备lewis细胞匀浆液,接种40只C57BL/6小鼠,随机分为空白组、环磷酰胺组、中药组、中药联合环磷酰胺组。观察15 d后,处死,剥离瘤组织,计算各用药组的抑瘤率;采用Elisa及RT-PCR检测方法,检测各组荷瘤小鼠瘤组织中P-gp、GSH表达水平的差异,评估其抗肿瘤多药耐药的疗效。结果：与空白组比较,各用药组中荷瘤小鼠体质量变化不明显（P〉0.05）,各用药组荷瘤小鼠平均瘤体质量明显降低,差异有统计学意义（P〈0.01）;与环磷酰胺组比较,中药联合环磷酰胺组及中药组的抑瘤率较高,差异有统计学意义（P〈0.01）;各用药组小鼠瘤组织中P-gp及GSH表达水平均低于空白组,差异有统计学意义（P〈0.01）;中药组及中药联合环磷酰胺组中P-gp的表达均低于环磷酰胺组,差异有统计学意义（P〈0.01）;中药联合环磷酰胺组中GSH的表达低于环磷酰胺组,差异有统计学意义（P〈0.01）。结论：化痰消瘤方可明显下调lewis肺癌荷瘤小鼠瘤组织中P-gp、GSH的表达水平,这可能是其逆转多药耐药的机制之一。

英文摘要：

Objective：To observe the effect of Huatan Xiaoliu Prescription on the expression of P-gp and GSH in tumor tissue of mice bearing lewis Lung cancer,and to explore the anti-multidrug resistance mechanism of it.Methods：Fifty C57BL/6 mice were inoculated with lewis cell homogenate and randomly divided into blank group,cyclophosphamide group,traditional Chinese medicine group and traditional Chinese medicine combined with cyclophosphamide group.The expression of P-gp and GSH in tumor tissue of tumor-bearing mice was detected by Elisa and RT-PCR.The differences of P-gp and GSH expression were observed and evaluated.Its multidrug resistance effect was evaluated.Results：Compared with that of the blank group,the body weight of the tumor-bearing mice of all other groups was not significantly changed（P〉0.05）.The average tumor mass of each tumor-bearing mice was significantly decreased（P〈0.01）.Compared with the cyclophosphamide group,the inhibitory rate of P-gp and GSH was significantly higher in the traditional Chinese medicine combined with cyclophosphamide group（P〈0.01）.The levels of P-gp and GSH in the tumor tissues of the mice in all medication groups were significantly lower than those in the control group（P〈0.01）.The expression of P-gp and GSH of the traditional Chinese medicine combined with cyclophosphamide group was significantly lower than that of the cyclophosphamide group（P〈0.01）.Conclusion：Huatan Xiaoliu Prescription can significantly downregulate the expression of P-gp and GSH in tumor tissue of Lewis Lung cancer mice,which may be one of the mechanisms of its reversing multidrug resistance effect.