中文摘要：

目的 观察细胞趋化因子CCL21及CC趋化因子受体7（CCR7）在过量碘和甲状腺球蛋白（Tg）免疫诱发的甲状腺炎中的表达,探讨二者在淋巴细胞浸润中的作用.方法 选用7～8周龄的雌性NOD小鼠,按体质量采用随机数字表法,将小鼠分为4组,每组7只.对照组：饮纯净水;Tg组：饮纯净水,使用猪的Tg加弗氏完全佐剂充分乳化后皮下免疫,8周龄时每只鼠注射0.1 mg Tg,在第11周龄和第15周龄分别加强免疫1次（剂量减半,加强免疫时用弗氏不完全佐剂）;高碘（HI）组：饮0.05％碘化钠（NaI）水;联合（HI＋ Tg）组：饮0.05％ NaI水,Tg免疫同Tg组.在无特定病原体（SPF）环境下专用饲料饲养.16周龄时处死全部小鼠并取甲状腺组织.HE染色观察甲状腺形态学改变,免疫荧光染色观察甲状腺中CD3、CD22、CCL21及CCR7的表达,实时荧光定量PCR法检测甲状腺组织内CCL21 mRNA的表达.结果 光镜下,HI组甲状腺滤泡扩张、胶质潴留,大量淋巴细胞浸润和结构破坏;Tg组有少量散在淋巴细胞浸润;HI＋ Tg组炎症反应和结构破坏加重.免疫荧光染色观察,浸润的淋巴细胞以CD3阳性的T淋巴细胞为主,仅有散在的CD22阳性的B淋巴细胞.其中,对照组未见CCL21和CCR7阳性细胞;Tg组仅见散在的CCR7阳性细胞;HI和HI ＋Tg组甲状腺炎细胞浸润区有CCL21强阳性表达,亦有CCR7阳性细胞聚集.CCL21 mRNA的相对表达水平：Tg组（3.20±0.18）、HI组（1.72±0.31）、HI＋ Tg组（9.94±0.32）较对照组（1.00±0.17）增加（P均＜0.01）.结论 趋化因子CCL21及其受体CCR7在自身免疫性甲状腺炎淋巴细胞浸润中有促进作用.

英文摘要：

Objective Through detecting the expression of chemokine CCL21 and its receptor CCR7 in thyroiditis of NOD mice induced by excessive iodine and thyroglobulin （Tg） immunization,to explore the mechanism of lymphocytic infiltration.Methods Seven to eight weeks old female NOD （non-obese diabetic） mice were used,the mice were divided into four groups by random number table,7 mice for each group.Control group：intoke distilled water;,Tg group：intoke＇distilled water,use pig Tg emulsified in Freund＇s complete adjuvant subcutaneous injection to immunization,0.10 mg Tg for each mouse at 8 weeks old,strengthened immunity at 11 and 15 weeks old （0.05 mg Tg for each mouse,and emulsified by Freund＇s incomplete adjuvant）; iodine excess （HI） group：intoke 0.05% NaI water; iodine excess combined with Tg （HI ＋ Tg） group：intoke 0.05% NaI water,Tg immunization method was same to Tg group; all mice were raised in specific pathogen free （SPF） environment.All mice were executed at 16 weeks old,and removed the thyroid tissue.Morphologic change in thyroid was observed by HE staining.Immunofluorescence （IF） staining was used to observe CD3,CD22,CCL21 and CCR7 expression in thyroid.Real-time PCR was used to detect CCL21 mRNA expression in thyroid.Results The morphologic change of thyroid was observed under the light microscope,in HI group,thyroid follicle distended,colloid accumulation,lymphocytes accumulate together and disorganization was seen; Tg group thyroid had scatted lymphocytes; HI ＋ Tg group enhanced inflammation response and disorganization.IF staining showed that lymphocytes infiltrated in thyroid were almost CD3＋ T cells,only few scatted CD22＋ B cells; CCL21 and CCR7 were negative in control group,CCR7 positive cells were scatted in Tg group but no CCL21 expression,in HI and HI ＋ Tg groups,we could see CCL21 strong positive expression in inflammation area and also had CCR7 positive cells there.CCL21 mRNA relative expression in thyroid：Tg group （3.20 ± 0.18）,HI group （1.