中文摘要：

目的 建立染料木素-4′-葡萄糖苷的含量测定方法。方法 采用反相高效液相色谱法。DiamonsilC18柱（4.6mm×250mm，5μm），流动相为乙腈-水（用磷酸调节pH至2.73）（25∶75），流速1.0mL·min^-1，检测波长261nm，柱温35℃。结果 染料木素-4′-葡萄糖苷在0.5055-101.1mg·L^-1内峰面积与浓度呈良好线性关系（r=1.0000，n=6），平均回收率99.2%（RSD=1.1%，n=9），日内精密度RSD〈0.5%，日间精密度RSD〈0.7%，最低检测限0.2ng。同时对样品中所含染料木素、染料木素-7-葡萄糖苷和染料木素-7，4′-二葡萄糖苷等微量有关物质的分离和检测也获得满意的结果。结论 该方法简便、可靠，可用于染料木素及其苷类的质量控制。

英文摘要：

OBJECTIVE To establish a HPLC method for determining the content of genistein-4＇-glucoside. METHODS The separation was performed on a Diamonsil C18 column （4.6 mm×250 mm,5 μm）. The mobile phase was acetonitrile-water （adjust pH to 2.73 by phosphoric acid） （25：75）. The flow rate was 1.0 mL · min^-1 and the detection wavelength was 261 nm. RESULTS The calibration curve was linear over the range of 0. 505 5 - 101.1 mg · L^-1 （ r = 1. 000 0, n=6） for genistein-4＇-glucoside. The aver- age recovery was 99. 2% （ RSD = 1.1% ,n =9）. The RSDs of intra-day and inter-day validatin were below 0. 5% and 0. 7% respectively. The LOD was about 0. 2 ng. The minor substances of genistein, genistein-7-glucoside and genistein-7-4＇-diglucoside in the sample also were isolated and detected satisfactorily. CONCLUSION The method is convenient and reliable for quality control of genistein and its related glycosides.