中文摘要：

一个 multistep 变换系统酚 hydroxylase 创作了(PH < 潜水艇 class= “ a-plus-plus ”> IND )和 2,3-dihydroxy-biphenyl 1,2-dioxygenase ( BphC <潜水艇class=“ a-plus-plus ”> LA-4 )被用来综合从o甲酚和m甲酚的 methylcatechols 和 semialdehydes 第一次。停靠 PyMOL 显示的研究预言甲酚和 methylcatechols 能被这个 multistep 变换系统理论上转变。高效液体层析团 spectrometry (HPLCMS ) 分析也显示从 multistep 变换形成的产品是相应 3-methylcatechol， 4-methylcatechol， 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoic 酸(2-hydroxy-3-methyl-ODA ) 和 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoic 酸(2-hydroxy-5-methyl-ODA ) 。recombinant E 的最佳的房间集中。表示酚 hydroxylase 的 coli 紧张 BL21 ( DE3 )( PH <潜水艇class=“ a-plus-plus ”> IND )和 2,3-dihydroxy-biphenyl 1,2-dioxygenase ( BphC <潜水艇class=“ a-plus-plus ”>为o甲酚和m甲酚的 multistep 变换的 LA-4 )和 pH 是 4.0 (接地模仿的 Ce ( IV )抽取出现了类似为高 Ce ( IV )集中和酸味大小写的趋势。模型结果显示高起始的 Ce (IV ) 集中和酸味两个都在 Ce (IV ) 抽取上有有害效果并且脱衣。在膜和散开层在之间的厚度的 Ce (IV ) 的散开系数喂阶段和膜阶段被获得，价值是 6.31 5 ?% 并且 9.0，分别地) ，但是没在降级上有效果新鲜， non-predegraded，文档(% BD？？

英文摘要：

A mulfistep conversion system composed of phenol hydroxylase （PHrND） and 2,3-dihydroxy-biphenyl 1,2-dioxygenase （BphCLA_4） was used to synthesize methylcatechols and semialdehydes from o- and m-cresol for the first time. Docking studies displayed by PyMOL predicted that cresols and methylcatechols could be theoretically transformed by this multistep conversion system~ High performance liquid chromatography mass spectrometry （HPLC-MS） analysis also indicated that the products formed from multistep conversion were the corresponding 3-methylcatechol, 4-methylcatechol, 2- hydroxy-3-methyl-6-oxohexa-2,4-dienoic acid （2- hydroxy-3-methyl-ODA） and 2-hydroxy-5-methyl-6-oxo- hexa-2,4-dienoic acid （2-hydroxy-5-methyl-ODA）. The optimal cell concentrations of the recombinant E. coli strain BL21 （DE3） expressing phenol hydroxylase （PHrND） and 2,3-dihydroxy-biphenyl 1,2-dioxygenase （BphCLA_4） and pH for the multistep conversion of o- and m-cresol were 4.0 （g-L-1 cell dry weight） and pH 8.0, respectively. For the first step conversion, the formation rate of 3- methylcatechol （0.29μmol·L-1·min-1·mg-1cell dry weight） from o-cresol was similarly with that ofmethylca- techols （0.28 μmol·L-1·min-1·mg-1 cell dry weight） from m-cresol by strain PHrND. For the second step conversion, strain BphCLA_4 showed higher formation rate （0.83 μmol·L-1·min-1·mg-1 cell dry weight） for 2-hydroxy-3-methyl- ODA and 2-hydroxy-5-methyl-ODA from m-cresol, which was 1.1-fold higher than that for 2-hydroxy-3-methyl- ODA （0.77 μmol·L-1·min-1·mg-1. mglcell dry weight） from ocresol. The present study suggested the potential application of the multistep conversion system for the production of chemical synthons and high-value products.