中文摘要：

【目的】分析家蚕感染质型多角体病毒（Bombyx mori cytoplasmic polyhedrosis virus,BmCPV）后中肠组织中蛋白质的差异表达,为从蛋白质分子水平上探索家蚕感染BmCPV后的分子应答机制奠定基础。【方法】通过蛋白质双向凝胶电泳技术（two-dimensional polyacrylamide gel electrophoresis,2-DE）对BmCPV病毒感染组和灭菌水对照组家蚕p50的中肠组织蛋白进行比较,通过基质辅助质量飞行时间质谱技术（MALDI-TOFMS）和家蚕蛋白质数据库检索对所得到的差异蛋白点进行鉴定与分析。【结果】2-DE电泳结果比较分析表明,BmCPV感染组和对照组家蚕的中肠组织有8个差异蛋白斑点,其中对照中肠有3个,感染中肠有5个。经MALDI-TOF MS鉴定结果显示对照家蚕中肠中的3个差异蛋白斑点分别为家蚕的类固醇脱氢酶（Hydroxysteroid dehydrogenase）、线粒体电压依赖的阴离子通道孔蛋白（Voltage-dependent anion-selective channel,VDAC）和1个未知新型蛋白;感染家蚕中肠的5个差异蛋白点分别是家蚕的Ras-鸟嘌呤核苷酸释放因子1（Ras-specific guanine nucleotide-releasing factor 1-like,RASGRF1）、H＋转运ATP合成酶亚基、候选肿瘤抑制因子（Putative tumor suppressor protein,PDSS2）、多药耐药蛋白（Multidrug resistance-associated protein,MRP4/ABCC4）、冻坏B样蛋白（Nipped-B-like protein-like,NIPBL）。【结论】BmCPV感染可导致家蚕中肠组织多种蛋白的差异表达,提示家蚕可能通过启动不同的机制来应答病毒的感染,其中VDAC的下调表达以及PDSS2、MRP4/ABCC4和NIPBL的上调表达均与诱导细胞凋亡相关,推测家蚕在BmCPV感染的过程中可能启动了细胞凋亡的抗病毒机制。

英文摘要：

[Objective] The objective of this study is to analyze differentially expressed proteins in the midgut of silkworm strain pS0 induced by Bombyx mori cytoplasmic polyhedrosis virus （BmCPV）, and to explore the immune mechanism of silkworm against BmCPV infection, which would also provide valuable information for further study. [Method] The differentiated proteins in the midgut of silkworm which induced by BmCPV was investigated by two-dimensional polyacrylamide gel electrophoresis （2-DE）, and the characteristics and functions of the differentially expressed proteins were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry （MALDI-TOF MS） and Silkworm Protein Data Bank. [ Result ] Between the control and BmCPV infected silkworm midgut, 8 differentiated protein spots （3 in control and 5 in BmCPV infected silkworm） were distinguished. In the control silkworm midgut, the three specific spots were hydroxysteroid dehydrogenase, voltage-dependent anion-selective channel （VDAC） and a new unknown protein. However, in the BmCPV infected silkworm midgut, the five specific spots might be ras-specific guanine nucleotide-releasing factor l-like （RASGRF1）, H＋ transporting ATP synthase beta subunit isoform 1, putative tumor suppressor protein （PDSS2）, multidrug resistance-associated protein （MRP4/ABCC4） and nipped-B-like protein （NIPBL）. [Conclusion] BmCPV infection induced a variety of differentially expressed proteins in the midgut, suggesting that different mechanisms were activited in the silkworm to response to the virus infection. Of the 8 differnentiated proteins, the down-regulated expression of VDAC and the up-regulated expression of PDSS2, MRP4/ABCC4 and NIPBL could respectively induce apoptosis. It would provide clues for elucidating the apoptosis program of silkworm against BmCPV infection.