中文摘要：

用荧光光谱法和UV光谱法,研究染料荧光素、铬黑T、结晶紫（Crystal Violet）与牛血清白蛋白（BSA）的作用,可以给出它们在不同结合数下的生成常数,求出它们的能量转移效率、给体-受体之间的距离,进而得知一些小分子染料与蛋白质结合后所引起染料或蛋白质光谱特性的变化.因此,这些小分子染料可作为蛋白质的探针,从分子水平的角度研究蛋白质与小分子相互作用的机理,从而为生命科学研究以及药物研究提供有价值的信息.

英文摘要：

In this article,the interactions mechanism of dye Fluorescein,Eriochrome Black T and Methyl Violet with Bovine Serum Albumin have been studied by means of fluorescence spectrum and UV spectrum,and the association constant with different binding numbers has also been obtained;Furthermore,we have also got the energy transferring efficiency and the distance of donor and acceptor.Thus we can understand the interactions mechanism of protein and small molecule from the perspective of molecular level and then provide useful information for life science research and medicine research