中文摘要：

目的观察脑红蛋白（NGB）在足月健康新生儿和宫内窘迫新生儿血清中的水平，探讨宫内缺氧对血清NGB表达的影响及NGB作为早期判断宫内缺氧检测指标的价值。方法建立血清NGB的双抗体夹心ELISA检测方法，获得稳定的标准曲线，检测35例（男17例，女18例）足月健康新生儿（健康对照组）NGB水平，分析不同性别间血清NGB表达水平的差异，初步确定足月健康新生儿血清NGB水平；同样方法检测32例宫内窘迫新生儿（宫内窘迫组）出生时脐动脉血清NGB水平，与足月健康新生儿比较。采用Stata7．0软件进行统计学分析。结果获得双抗体夹心ELISA拟和标准曲线方程为Y=exp（-5．881532＋11．955890×X）＋2．281506E-02（r=0．9994P〈0．001）。健康对照组新生儿出生时脐动脉血清NGB水平为（27．35±4．41）μg／L，男女NGB水平比较无差异（t=0．4187P〉0．05）；宫内窘迫组新生儿脐动脉血清NGB水平为（149．7±43．2）μg／L，显著高于健康对照组新生儿（t=2．9416P〈0．01）。结论新生儿脐动脉血清NGB水平作为判别HIE的早期检测指标，具有临床应用价值。

英文摘要：

Objective To observe the change of serum neuroglobin （ NGB ） of normal full - term and fetal distress infants, and to explore the sensitivity and validity of NGB as potential biomarker for brain injury. Methods The technique of double - antibody sandwich enzyme - linked immunosorbent assay to measure NGB was established, and stable data standard curve was gained. Serum NGB of umbilical cord artery in 35 normal full - term （ 17 male, 18 female）and 32 newborn infants were measured. The differece of serum NGB in gender, and the changes in serum NGB of fetal distress infants were analyzed,who were probably injured by anoxia. The results were analyzed by Stata 7.0 software. Results The standard curvilinear equation was Y = exp（ - 5.881 532 ± 11. 955 890 × X） ＋ 2. 281 506E - 02 （ linear correlation coefficient r = 0. 999 4 P 〈 0. 001 ） ;the average NGB level in serum of umbilical cord artery of normal full - term infants was（ 27.35 ± 4.41 ） μg/L, and no difference in gender（ t = 0. 418 7 P 〉 0.05 ） ; the average NGB in the serum of umbilical cord artery of fetal distress infants was （ 149.7 ± 43.2 ） μg/L, which was significantly higher than that of normal full - term infants （ t = 2.941 6 P 〈 0.01 ）. Conclusion The sensitivity and validity of serum NGB as a potential biomarker for hypoxic brain injury need further evaluation.