以我国西南地区3个常用大豆品种为材料,比较净作和套作条件对大豆籽粒发育及后熟过程中异黄酮积累规律的影响。结果表明:籽粒成熟期按总异黄酮积累速率可分为初始积累期和快速积累期;受田间小气候影响,套作条件下大豆较晚进入快速积累期;田间采收时,贡选1号品种净作总异黄酮含量极显著高于套作,南豆16和贡秋豆3号净作与套作之间差异不显著。3个品种籽粒后熟期套作条件下总异黄酮的积累量均极显著高于净作,总异黄酮含量套作均显著高于净作;后熟期不同品种异黄酮各组分及总异黄酮的积累量均存在显著差异。本研究发现净套作对3个大豆品种的异黄酮积累动态及总含量均有显著影响,品种间籽粒异黄酮的积累对种植条件的反应不同。相对净作而言,后熟过程更利于套作大豆籽粒异黄酮的进一步积累。
Seed isoflavone accumulation pattern at seed developing stage and during after-ripening process of soybean were studied under sole cropping ( SC) and relay cropping ( RC) condition. According to isoflavone accumulation rate,the seed development stage could be divided into two periods,initial accumulation period and fast accumulation period. However,affected by the field microclimate,the fast accumulation period of RC seeds came later than that of SC seeds. At harvest time,the seed isoflavone content of Gongxuan 1 cultivar under SC was significantly higher than that of RC. During the after-ripening period,isoflavone content of all three varieties under RC condition was significantly higher than that under SC condition. Furthermore,there were significant differences in the accumulation of malonylglucosides,glucosides and total isoflavones among the three varieties at this period. The results indicated that two cropping patterns have significant influence on isoflavone accumulation,although there were different responses to cropping patterns among soybean cultivars. The after-ripping process was beneficial to seed isoflavone accumulation of soybean especially in the RC soybean seeds.