中文摘要：

提高的外部 counterpulsation (EECP ) 改进的背景在有倔强的心绞痛的病人的局部缺血，而是机制仍然保持不清楚。探索 EECP 行动的机制，我们检测了介绍任何下列标记 CD34+ ， CD29+ ，和 CD106+.Methods 生长调停 cytokines 的祖先房间的祖先房间动员和联系 angiogenesis 潜力在血胆脂醇过多的一个猪的模型被估计。24 头男国内猪随机被分到 4 个组：正常饮食(控制， n=6 ) ， hypercholesterolemic 饮食(CHOL， n=6 ) ，有 recombinant 人 granulocyte 的管理的 hypercholesterolemic 饮食刺激殖民地的因素(rhG-CSF )(rhG-CSF， n=6 ) ，并且有 EECP 治疗的 hypercholesterolemJc 饮食(EECP， n=6 ) 。EECP 被使用 2 个小时为 36 个小时的一个总数的每隔一天。脉管的 endothelial 生长因素( VEGF )和刺激殖民地的因素( G-CSF )，外部血祖先细胞计数，地区性的 angiogenesis 的水平，和 VEGF 和 stromal 房间的表示导出的 granulocyte 的浆液层次( SDF-1 )因素 1 在猪的心肌层被估计， respectively.Results 导致血胆脂醇过多的动脉硬化的一个猪的模型成功地被建立。在在四个组之中的 VEGF 的浆液层次没有重要差别。在 EECP 组的 G-CSF 的浆液层次在星期 15 和星期 18 点显著地增加了((38.3

英文摘要：

Background Enhanced external counterpulsation （EECP） improves ischemia in patients with refractory angina pectoris but the mechanism remains unclear. To explore the mechanisms of EECP action, we detected progenitor cells presenting any of the following markers CD34^＋, CD29^＋, and CD106^＋. Methods Growth cytokines-mediated progenitor cell mobilization and associated angiogenesis potential were assessed in a porcine model of hypercholesterolemia. Twenty-four male domestic swines were randomly assigned to 4 groups： normal diet （control, n=6）, hypercholesterolemic diet （CHOL, n=-6）, hypercholesterolemic diet with administration of recombinant human granulocyte colony-stimulating factor （rhG-CSF） （rhG-CSF, n=6）, and hypercholesterolemic diet with EECP treatment （EECP, n=6）. EECP was applied 2 hours every other day for a total of 36 hours. Serum levels of vascular endothelial growth factor （VEGF） and granulocyte colony-stimulating factor （G-CSF）, peripheral blood progenitor cell counts, level of regional angiogenesis, and expression of VEGF and stromal cell derived factor l a （SDF-1α） in porcine myocardium were assessed, respectively. Results A porcine model of hypercholesterolemia-induced arteriosclerosis was successfully established. There was no significant difference in serum levels of VEGF among the four groups. The serum levels of G-CSF in the EECP group increased significantly at week 15 and week 18 （（38.3±5.6） pg/ml at week 15 vs （26.2±3.7） pg/ml at week 12, P 〈0.05, and （46.9±6.1） pg/ml at week 18 vs （26.2±3.7） pg/ml at week 12, P 〈0.01）. The serum levels of G-CSF in group 3 increased also significantly after receiving rhG-CSF injection for five days （（150±13.9） pg/ml at week 18 vs （24.8±5.4） pg/ml at week 12, P 〈0.01）. Compared to other groups and other time points, progenitor celt counts increased significantly after 2-hour EECP treatment （108±13 vs 26±6 per 10^5 leukocytes, P 〈0.01）, but not at week 18. The