利用氨基酸自动分析仪对滞育和滞育后越北腹露蝗卵血淋巴中的游离氨基酸进行分析.结果表明,共发现31种游离氨基酸.滞育解除后游离氨基酸总含量增加,但精氨酸、鸟氨酸、γ-氨基丁酸、胱硫醚和瓜氨酸的含量却显著下降,比滞育期分别减少88.99%、76.15%、78.15%、100.00%和100.00%.游离氨基酸总含量的增加可能是用于合成胚胎发育所需的蛋白质;而精氨酸,鸟氨酸,γ-氨基丁酸,胱硫醚,瓜氨酸含量的下降意味着滞育即将解除.滞育期间,精氨酸、鸟氨酸、丝氨酸、脯氨酸、赖氨酸、丙氨酸、缬氨酸保持着较高的含量,说明这些氨基酸可能是重要的耐寒物质.与多数滞育昆虫不同的是,精氨酸在滞育期含量最高,脯氨酸在滞育后的胚胎发育期含量最高,表明具有专性滞育特性的越北腹露蝗有着自己独特的氨基酸代谢机制.
The free amino acids in the haemolymph of diapause and postdiapause egg of the grassshopper, Fruhstorferiola tonkinensis, were investigated by an automatic amino-acid analyzer. Thirty-one amino acids were detected in the haemolymph of two studied stages. The postdiapause of the grasshopper resulted in an increase in content and kinds of free amino acids except arginine ( - 88. 99% ), ornithine ( -76. 15% ) , T-amino-n-butyric acid ( - 78. 15% ), cystathionine ( - 100. 00% ) and citruUine ( -100. 00% ), which indicated that the postdiapause eggs had significantly elevated essential amino acid content related to protein synthesis for embryonic development and decreased arginine, ornithine, γ- Amino-n-butyric acid, cystathionine and citrulline to terminate diapause. Arginine, ornithine, serine, proline, lysine, alanine and valine remaining high contents during the diapause duration may be the main antifreezes for the overwintering eggs of the grasshopper. Compared to other diapause insects, however, the contents of arginine and proline of the diapause and postdiapause egg of grasshopper were the highest respectively, and indicated that the grasshopper had its special metabolism of amino acids for its diapause.