中文摘要：

多孔板-MTT比色法测定植物抗菌成分对细菌抑制活性的步骤为：每孔加入浓度为10^6cfu／mL的供试菌液90灿，然后加入不同浓度的药液10μL，28℃暗培养24h后，每孔中加入5mg／mL的MTT溶液10μL，继续培养4h后加入10％二甲基亚砜100μL，振荡30min，在570nm处测定溶液的吸光值。采用以上方法，测定植物抗菌成分蓝桉醇对辣椒斑点病黄单胞菌（Xanthomonas vesicatoria）和枯草芽孢杆菌（Bacillus subtilis）的半抑制浓度（IC50）分别为0．158和0．395mg／mL，小檗碱对溶血葡萄球菌（Staphylococcus haemolyticus）的IC50为0．587mg／mL。结果表明，多孔板-MTT比色法能快速、微量地测定植物成分对细菌的抑制活性。

英文摘要：

An optimal experimental procedure with microplate-MTT colorimetric assay was obtained ,that was ： 1 ） addition of 90 μL of bacterial suspension in concentration of 106 cfu/mL in each well of a 96-well micro plate ;2） then addition of 10 μL of the component solution with different concentration ;3 ） cultivation for 24 h at 28 0℃ in darkness ;4） then 10 μL of MTT solution in 5 mg/mL was added in each well;5） after incubation for another 4 h at 28 ℃ ,100 μL of 10% DMSO was added;6） after shaking for another 30 min ,light absorption values were obtained at 570 nm for the snpernatant with a micro plate spectrophotometer. As the optimal MTT assay mentioned above, globulol from Eucalyptus globulus fruits showed strong inhibitory activities against Xanthomonas vesicatoria and Bacillus subtilis that median effective inhib- itory concentration（ IC50 ） were 0. 158 and 0. 395 mg/mL respectively. Berberine from Phellodendron chinertse fruits had its strong inhibitory activity against Staphylococcus haemolyticus obtaining 0. 587 mg/mL of IC50. This indicated that plant antibacterial components could be evaluated fast and micro-quantitatively by using microplate-MTT colorimetric assay.