乙烯信号途径的建立得益于一系列的突变体研究,EIN3是乙烯信号转导通路的核心转录因子,EIN3的蛋白质含量严格受F-BOX蛋白EBF1/EBF2的降解调控。为了进一步挖掘乙烯信号途径的新组分和深入研究EIN3及其下游的信号组分,作者筛选了四个不同来源的T-DNA库,并利用转基因植物EIN3ox作为遗传背景,进行了EIN3下游的抑制子筛选工作,还利用化学遗传学的方法筛选了四个小分子库。
Genetic and biochemical study of mutants involved in ethylene signaling established the current molecular framework of ethylene signaling in Arabidopsis.EIN3 as a key transcription factor is negatively regulated by EBF1/2 mediated degradation.To discover new components involved and to further study the events downstream EIN3,we screened mutants from four T-DNA insertion mutants? pools and another EMS mutated pool in background of EIN3ox.Besides,we screened four small compounds pools for chemicals may have effect on ethylene signaling in plants.