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中文摘要：
根据鸡肽转运载体cPepT1 mRNA和看家基因β-actin mRNA序列，分别设计cPepT1和β-actin引物各1对，并通过Mg^2＋浓度、循环数等PCR条件的优化，建立了包括RNA提取、反转录、PCR的鸡小肠cPepT1基因表达检测方法。采用该方法研究了肉仔鸡cPepT1基因在小肠各段的表达差异。结果显示，肉仔鸡小肠cPepT1表达水平表现为随小肠近端到远端显著降低的趋势，回肠表达水平显著低于十二指肠和空肠，十二指肠和空肠差异不显著。
英文摘要：
of semi-quantity RT-PCR was established to evaluate gene expression of peptide transporter in small intesfine of chick. The primers of β-actin and cPepT1 were designed according to their gene sequence reported. The cycles and Mg^2＋ concentrations were optimized by experiment. The results showed that gene expression of cPepT1 varied in intestinal segments, decreased from the proximal to the distal, i.e. the level of the expression ofcPepT1 in ileum was higher than that in duodenum and jejunum significantly, no difference was found between duodenum and jejunum.
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