中文摘要：

在室温、p H=7.4、10 mmol/L 4-羟乙基哌嗪乙磺酸（Hepes）缓冲溶液条件下,利用荧光光谱研究了卵清蛋白（Ova）与Tb^（3＋）的结合性质.利用荧光探针2-对甲苯胺基-6-萘磺酸钠（TNS）检测了Tb^（3＋）对Ova疏水区的影响.通过化学变性实验分析了Ca2＋和Tb^（3＋）对Ova结构稳定性的影响.结果表明,Ova可以与Tb^（3＋）形成1∶2的复合物,Tb^（3＋）的结合使其疏水区暴露程度增加,TNS的荧光增强.Ova的解折叠曲线呈现三态的变化（N■■I■■U）.Ca2＋可以增加I■■U过程的稳定性,Tb^（3＋）对N■■I和I■■U两个解折叠过程都有影响.

英文摘要：

The binding properties of Tb~（3＋）with ovalbumin were measured by fluorescence spectra at room temperature in p H = 7. 4,10 mmol / L 4-（ 2-hydroxyethyl）-1-piperazinecthanesulfonic acid（ Hepes）. The effect of Tb~（3＋）on the hydrophobic region of ovalbumin was measured by hydrophobic probe 2-p-toluidino-6-naphthalenesalfonic acid（ TNS）. The effects of Ca2＋and Tb~（3＋）on the stability of ovalbumin were analyzed by chemical unfolding methods. The results suggested that ovalbumin can form 1 ∶ 2 complexes with Tb~（3＋）. The binding of Tb~（3＋）increased the hydrophobic surface of ovalbumin,and the fluorescence intensity of TNS increased. The unfolding curves of ovalbumin showed two transition,N■■I and I■■U. Ca2＋can increase the stability of transition I■■U and Tb~（3＋）can increase the stability of transitions N■■I and I■■U.