中文摘要：

目的：观察重组人内抑素对佐剂性关节炎大鼠滑膜组织血管内皮细胞生长因子（vascular endothelial cell growth factor,VEGF）的表达及其作用.方法：采用弗氏完全佐剂（Freund＇s complete adjuvant,CFA）诱导的佐剂性关节炎 （adjuvant-induced arthritis,AA） 大鼠模型,用足容积法测量关节肿胀度;免疫组织化学法检测血管内皮细胞生长因子的表达及其对微血管密度的影响.结果：弗氏完全佐剂致炎后 d 10,佐剂性关节炎大鼠出现继发性炎症,皮下注射给予不同剂量的重组人内抑素（1.25、2.5、5 mg·kg^-1）,连续 7 d,对照组于 d 10 给予氨甲喋呤（Methotrexate,MTX,1.0 mg·kg^-1）.重组人内抑素各剂量组能明显抑制AA大鼠的继发性足肿胀,重组人内抑素各剂量组可不同程度减少AA大鼠关节滑膜组织高表达的VEGF,还可使滑膜组织的微血管密度减少.结论：重组人内抑素可显著抑制AA大鼠滑膜组织VEGF的表达及微血管密度,该作用可能与抑制血管翳的生成及减少滑膜细胞表面VEGF的表达有关.

英文摘要：

AIM：To investigate the effect of recombinant human endostatin on the expression of vascular endothelial cell growth factor （VEGF） in adjuvant arthritis synovium tissue. METHODS： Adjuvant arthritis rat model was induced by a single intradermal injection of 100 μl of Freund＇ s complete adjuvant（CFA）on d 0. The volume of rat hind paw was measured by means of Volume Meter. The expression of VEGF and microvessel density of the synovial tissue were measured by immunohistochemical method. RESULTS： The secondary inflammation of Adjuvant-induced arthritis（AA） rats appeared on the d 10 after the injection of CFA. The therapeutic administration of recombinant human endostatin（ 1.25,2.5,5.0 mg·kg^-1 ） were given for 7 days consecutively. Methotrexate （MTX） （1.0 mg·kg^-1）was given only once on d 10 as the control. It was found that recombinant human endostatin significantly inhibited the secondary paw swelling. Recombinant human endostatin significantly decreased the expression of VEGF and microvessel density of the synovial tissue on AA rots. CONCLUSION： Recombinant human endostatin had an inhibitory effect on the expression of VEGF and microvessel density of the synovial tissue on AA rats, which was related to its anti-angiogenesis and an inhibition of VEGF on synoviocytes.