中文摘要：

目的 观察中链甘油三酯（medium-chain triglyceride,MCT）对高胆固醇血症小鼠体质量、体脂、血清脂质水平及其体内胆固醇逆转运的影响.方法 建立高胆固醇血症小鼠模型,将30只造模成功小鼠随机分为中链甘油三酯组（MCT组）和长链甘油三酯（long-chain triglyceride,LCT）组,给予含2％ MCT和2％ LCT高脂高胆固醇饲料喂养3个月后,腹腔注射富含3H胆固醇的RAW264.7巨噬细胞悬液,单笼饲养3d,收集每天粪便,腹主动脉取血,测量体长、肝及附睾脂肪组织.测定血清脂质,用液闪计数仪测定血清、肝及粪便中3H胆固醇放射活性.结果 实验结束时,与LCT组相比,MCT组小鼠空腹体质量、体质量增加量、Lee氏指数、附睾脂肪重量及血清TC水平显著降低（P＜0.05）;两组血清HDL-C、n-HDL-C和TG差异无统计学意义（P＞0.05）;与LCT组比较,MCT组血清和肝3H胆固醇水平均显著降低（P＜0.05）,同时48 h粪便中3H胆固醇流出量及72 h总量显著增加（P＜0.05）.结论 MCT能够减轻高胆固醇血症小鼠体质量,减少体脂肪积累和改善胆固醇代谢,且能促进体内胆固醇的逆转运.

英文摘要：

Objective To investigate the effects of medium-chain triglyceride （MCT） on reverse cholesterol transport in mice with hypercholesterolemia.Methods Thirty mice with hypercholesterolemia induced by feeding high-fat and cholesterol were randomly divided into 2 groups （MCT group and LCT group,n=15）,and they were fed high-fat and cholesterol diets with 2％ MCT or 2％ long-chain triglyceride （LCT）,respectively.Three months later,macrophage reverse cholesterol transport （RCT） was assessed by injecting 3H-cholesterol labeled RAW264.7 macrophages intraperitoneally.After 3 days,feces were collected and the body length,liver and epididymides tissues were measured.The serum lipid was measured and the activity of 3H-tracer in serum,liver and feces were determined by liquid scintillation method.Results At the end of study,the body weight,weight gain,liver weight,Lee＇s index,epididymides fat weight and serum levels of TC were significantly lower in MCT group than those in LCT group （P ＜ 0.05）,while no significant difference showed in serum levels of HDL-C,n-HDL-C and TG between the two groups （P ＞ 0.05）.Compared with the LCT group,the 3H-cholesterol in serum and liver were much lower in MCT group （P ＜ 0.05）,while the total 3H-cholesterols in feces in 3 days experiment and at 48h after the injection of 3H-cholesterol labeled RAW264.7 macrophages increased significantly （P ＜ 0.05）.Conclusion MCT can reduce the body weight and body fat,and it also can improve cholesterol metabolism and promote RCT in mice with hypercholesterolemia.