应用形态学、解剖学及组织学方法对玻璃扯旗鱼的性别特征及其幼鱼性别分化基本过程进行研究。结果显示:在6月龄玻璃扯旗雌鱼卵巢中能观察到各时相的卵母细胞,在雄鱼精小管管腔内有大量精子;4月龄以上玻璃扯旗雌鱼的腹部隆起,并有淡黄色颗粒状组织,而雄鱼无类似结构;100日龄以上雌鱼的臀鳍有钩状小齿,但2月龄前的幼鱼无该组织。5~50日龄幼鱼性腺发育状态显示:10日龄以下幼鱼的体内无分化明显的生殖腺;15日龄幼鱼体内有生殖包囊,且15日龄之前仅在腹腔一侧可观察到性腺组织,而25日龄幼鱼的腹腔两侧均有对称分布的类卵巢结构;至30日龄时,部分幼鱼可观察到有棒状精巢,性腺开始分化。本研究中探明了玻璃扯旗鱼性别分化及性腺发育的关键时间节点,该结果可为进一步研究其分子调控机制提供参考。
Analysis on sex differentiation and gonadal development of Pristella maxillaris was conducted in the study bymorphological,anatomical and histological methods.The results showed that different stages of oocytes could beobserved at6–month–old female fish,and a large number of sperm filled in seminiferous tubules of male testis.Thesexual characteristics emerged with appearance of yellow granular structure in abdomen or the uplifted abdomen whenfishes were over4–month–old.Hook–shaped teeth were also observed in the anal fins of fishes over100– day–old age,however,they could not be observed when fishes were in less two months.Based on the analysis from the gonads in5to50day–old fishes,it showed that there was no differentiated gonads in both sides of dorsal abdominal cavity at10dayspost–fertilization(dpf);later,there was germline cysts found in the abdominal cavity at15dpf,and the gonadal tissuewas only spotted at one side of the abdominal cavity before15days.The ovary–like structure(‘juvenile ovary’)symmetrically appeared at25dpf and the baculiform testis appeared at30dpf.All these facts could provide a furtherstudy reference for sex differentiation,gonadal development and their regulative mechanism.