为探索提高氮素利用率的途径,采用盆栽方法研究了两个不同氮效率小麦品种籽粒蛋白质形成的差异及不同形态氮素对其的影响。结果表明,高氮吸收型小麦品种秦麦11灌浆期籽粒GS、GPT活性、蛋白质及其组分含量均大于低氮吸收型小麦品种扬9817。不同氮素形态对两个不同氮效率小麦品种籽粒蛋白质及其动态变化、蛋白组分含量及其动态变化的影响不同。高氮吸收型小麦品种秦麦11籽粒蛋白质及其组分含量均表现为酰胺态氮处理〉铵态氮处理〉硝态氮处理。处理间蛋白质含量、球蛋白含量、谷蛋白含量的差异达到显著水平。低氮吸收型小麦品种扬9817籽粒蛋白质含量、清蛋白含量、球蛋白含量、醇溶蛋白含量均表现为酰胺态氮处理〉硝态氮处理〉铵态氮处理,谷蛋白含量在酰胺态氮处理下最高,硝态氮处理下最低,处理间清蛋白含量、醇溶蛋白含量、谷蛋白含量的差异达到显著水平。
In order to improve the N efficiency, pot experiments were carried out to study the differenee in grain protein formation between two wheat varieties with different N efficiency and the regulation effects of N form. The results indicated that the activities of GS and GPT, contents of protein and its components in grains of Qinmai11 with high N efficiency were greater than those of Yang 9817 with low N efficiency. The effects of N forms on contents of protein and protein components in grains were different between two wheat varieties with different N efficiency. To Qinmai11 with high N efficiency, the order of contents of protein and protein components in grains among N form treatments was CONH2-N〉NH4^+-N〉NO3^--N, the difference in contents of protein, globulin and glutenin in grains among three different N form treaments was significant. To Yang 9817 with low N efficiency, the order of contents of protein, albumin, globulin, gliadin in grains among N form treatments was CONH2-N〉NO3^--N〉NH4 +-N, and glutenin content was the highest in CONH2-N treatment, while it was the lowest in NO3^--N treatment, the difference of contents of albumin, gliadin and glutenin in grains among three different N form treatments was significant.